Product Name :
Histone H3 (A1) polyclonal antibody Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Histone H3 (A1) polyclonal antibody detects endogenous levels of Histone H3 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Histone H3. Conjugate :
Unconjugated Modification :
Unmodification
Histone H3 (A1) polyclonal antibody Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Histone H3 (A1) polyclonal antibody detects endogenous levels of Histone H3 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Histone H3. Conjugate :
Unconjugated Modification :
Unmodification
-
-
Immunohistochemistry (IHC) analyzes of Histone H3 (A1) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Potential role of microRNA-7 in the anti-neuroinflammation effects of nicorandil in astrocytes induced by oxygen-glucose deprivation
PMCID: Pubmed No.:26961366
Hydrogen Sulfide Induces Keap1 S-sulfhydration and Suppresses Diabetes-Accelerated Atherosclerosis via Nrf2 Activation
PMCID: Pubmed No.:27335232
PPARγ agonists regulate bidirectional transport of amyloid‐β across the blood–brain barrier and hippocampus plasticity in db/db mice
PMCID: Pubmed No.:26507867
Preventive effect of genetic knockdown and pharmacological blockade of CysLT1R on lipopolysaccharide (LPS)-induced memory deficit and neurotoxicity in vivo
PMCID: Pubmed No.:27810377
LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway
PMCID: Pubmed No.:26418365
Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer
PMCID: Pubmed No.:25546612
CD49f promotes proliferation of male dairy goat germline stem cells
PMCID: Pubmed No.:26841372
Triethylenetetramine Synergizes with Pharmacologic Ascorbic Acid in Hydrogen Peroxide Mediated Selective Toxicity to Breast Cancer Cell
PMCID: Pubmed No.:28280522
Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3β/β-catenin integrated signaling pathway
PMCID: Pubmed No.:28408345
17beta-estradiol promotes the odonto/osteogenic differentiation of stem cells from apical papilla via mitogen-activated protein kinase pathway
PMCID: Pubmed No.:25403930
Targeted inhibition of RAGE reduces amyloid-β influx across the blood-brain barrier and improves cognitive deficits in db/db mice
PMCID: Pubmed No.:29248482
CysLT1R downregulation reverses intracerebroventricular streptozotocin-induced memory impairment via modulation of neuroinflammation in mice
PMCID: Pubmed No.:27720931
Montelukast ameliorates streptozotocin-induced cognitive impairment and neurotoxicity in mice
PMCID: Pubmed No.:27702591
Syndecan-4 Signaling Is Required for Exercise-Induced Cardiac Hypertrophy
PMCID: Pubmed No.:26835698
LYG-202 exerts antitumor effect on PI3K/Akt signaling pathway in human breast cancer cells
PMCID: Pubmed No.:26153346
Deletion of Brg1 causes abnormal hair cell planer polarity, hair cell anchorage, and scar formation in mouse cochlea
PMCID: Pubmed No.:27255603
NOK mediates glycolysis and nuclear PDC associated histone acetylation
PMCID: Pubmed No.:28410146
Aspirin inhibits LPS-induced macrophage activation via the NF-κB pathway
PMCID: Pubmed No.:28912509
Protective effect of coptisine free base on indomethacin-induced gastric ulcers in rats: Characterization of potential molecular mechanisms
PMCID: Pubmed No.:29223540
Knockdown of hippocampal cysteinyl leukotriene receptor 1 prevents depressive behavior and neuroinflammation induced by chronic mild stress in mice
PMCID: Pubmed No.:26546369
Wogonoside inhibits angiogenesis in breast cancer via suppressing Wnt/β‐catenin pathway
PMCID: Pubmed No.:26387984
RAGE-NF-κB-PPARγ Signaling is Involved in AGEs-Induced Upregulation of Amyloid-β Influx Transport in an In Vitro BBB Model
PMCID: Pubmed No.:28871412
Simvastatin ameliorates memory impairment and neurotoxicity in streptozotocin-induced diabetic mice
PMCID: Pubmed No.:28499972
Anti-inflammatory activity of coptisine free base in mice through inhibition of NF-κB and MAPK signaling pathways
PMCID: Pubmed No.:28648405
Ethanol exposure represses osteogenesis in the developing chick embryo
PMCID: Pubmed No.:27112526
Ursolic Acid Ameliorates Early Brain Injury After Experimental Traumatic Brain Injury in Mice by Activating the Nrf2 Pathway
PMCID: Pubmed No.:27734181
PPARδ agonist GW0742 ameliorates Aβ 1–42 -induced hippocampal neurotoxicity in mice
PMCID: Pubmed No.:26864581
miR‐30d is related to asbestos exposure and inhibits migration and invasion in NCI‐H2452 cells
PMCID: Pubmed No.:28979837
Protective effect of melatonin on the development of abdominal aortic aneurysm in a rat model
PMCID: Pubmed No.:27392820
Integrin alphavbeta3 enhances β-catenin signaling in acute myeloid leukemia harboring Fms-like tyrosine kinase-3 internal tandem duplication mutations: implications for microenvironment influence on sorafenib sensitivity
PMCID: Pubmed No.:27248172
Prohibitin Protects Pulmonary Microvascular Endothelial Cells Against Cigarette Smoke Extract-Induced Cell Apoptosis and Inflammation
PMCID: Pubmed No.:35378837
PCAF Accelerates Vascular Senescence via the Hippo Signaling Pathway
PMCID: Pubmed No.:36246398
DIFFERENTIATED EMBRYONIC CHONDROCYTE EXPRESSED GENE-1 IS A CENTRAL SIGNALING COMPONENT IN THE DEVELOPMENT OF COLLAGEN-INDUCED RHEUMATOID ARTHRITIS
PMCID: Pubmed No.:36739947
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.