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Histone H3 (A1) polyclonal antibody BS3718
  • Immunohistochemistry (IHC) analyzes of Histone H3 (A1) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameHistone H3 (A1) polyclonal antibody
Catalog No.BS3718
Swiss-ProtP68431/Q71DI3/P84243
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000_x000D_ IHC: 1:50~1:200
BiowMW~ 17 kDa
Alternative NameHistone H3.1; H3/a; H3/b; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l;
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
Histone H3 (A1) polyclonal antibody
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Histone H3 (A1) polyclonal antibody detects endogenous levels of Histone H3 protein.
Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Histone H3.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Immunohistochemistry (IHC) analyzes of Histone H3 (A1) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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