Product Name :
Bcl-2 (phospho-S70) polyclonal antibody Background :
Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Bcl-2 (S70) polyclonal antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at Ser70. Immunogen :
Synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Serine 70. Conjugate :
Unconjugated Modification :
Phosphorylation
Bcl-2 (phospho-S70) polyclonal antibody Background :
Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Bcl-2 (S70) polyclonal antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at Ser70. Immunogen :
Synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Serine 70. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of Bcl-2 (phospho-S70) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 1h whole cell lysate(40ug) Lane3:HEK293T treated with UV for 6h whole cell lysate(40ug) Lane4:HEK293T treated with UV for 24h whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of p-Bcl2 (S70) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression
PMCID: Pubmed No.:26252661
MiR-449a regulates autophagy to inhibit silica-induced pulmonary fibrosis through targeting Bcl2
PMCID: Pubmed No.:27351886
MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression
PMCID: Pubmed No.:26252661
Long non-coding RNA ESCCAL-1 promotes esophageal squamous cell carcinoma by down regulating the negative regulator of APOBEC3G
PMCID: Pubmed No.:32905814
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.