Chk2 (phospho-T68) polyclonal antibody 
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Product Name :
Chk2 (phospho-T68) polyclonal antibody Background :
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Chk2 (T68) polyclonal antibody detects endogenous levels of Chk2 protein when phosphorylated at Thr68. Immunogen :
Synthetic phosphopeptide derived from human Chk2 around the phosphorylation site of Threonine 68. Conjugate :
Unconjugated Modification :
Phosphorylation
Chk2 (phospho-T68) polyclonal antibody Background :
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Chk2 (T68) polyclonal antibody detects endogenous levels of Chk2 protein when phosphorylated at Thr68. Immunogen :
Synthetic phosphopeptide derived from human Chk2 around the phosphorylation site of Threonine 68. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-Chk2 (T68) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with UV Lane2:Mouse kidney tissue lysate Lane3:H9C2 cell lysate treated with UV -
Immunohistochemistry (IHC) analyzes of p-Chk2 (T68) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Loss of KLF14 triggers centrosome amplification and tumorigenesis
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Induction of cell cycle arrest by GL331 via triggering an ATM-dependent DNA damage response in HepG2 cells
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Oroxylin A reverses P-glycoprotein-mediated multidrug resistance of MCF7/ADR cells by G2/M arrest
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HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation
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Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells
PMCID: Pubmed No.:25344109
LW‐213 induces G2/M cell cycle arrest through AKT/GSK3β/β‐catenin signaling pathway in human breast cancer cells
PMCID: Pubmed No.:25945460
Loss of KLF14 triggers centrosome amplification and tumorigenesis.
PMCID: Pubmed No.:26439168
Geminin Deletion in Mouse Oocytes Results in Impaired Embryo Development and Reduced Fertility
PMCID: Pubmed No.:26764091
Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells
PMCID: Pubmed No.:25344109
Loss of KLF14 triggers centrosome amplification and tumorigenesis.
PMCID: Pubmed No.:26439168
Geminin Deletion in Mouse Oocytes Results in Impaired Embryo Development and Reduced Fertility
PMCID: Pubmed No.:26764091
HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation
PMCID: Pubmed No.:24711370
Protein Phosphatase 6 Protects Prophase I-Arrested Oocytes by Safeguarding Genomic Integrity
PMCID: Pubmed No.:27930667
Attenuated LKB1-SIK1 signaling promotes epithelial-mesenchymal transition and radioresistance of non–small cell lung cancer cells
PMCID: Pubmed No.:27266881
LW‐213 induces G2/M cell cycle arrest through AKT/GSK3β/β‐catenin signaling pathway in human breast cancer cells
PMCID: Pubmed No.:25945460
Geminin deletion in pre-meiotic DNA replication stage causes spermatogenesis defect and infertility
PMCID: Pubmed No.:28690291
Rad9a is involved in chromatin decondensation and post-zygotic embryo development in mice
PMCID: Pubmed No.:30154445
Ablation of beta subunit of protein kinase CK2 in mouse oocytes causes follicle atresia and premature ovarian failure
PMCID: Pubmed No.:29725001
CHEK2 signaling is the key regulator of oocyte survival after chemotherapy
PMCID: Pubmed No.:37862420
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.