Product Name :
GSK3α/β (phospho-Y279/216) polyclonal antibody Background :
Glycogen synthase kinase-3 α and β (GSK-3α, β) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-GSK3α/β (Y279/216) polyclonal antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Tyr279/216 Immunogen :
Synthetic phosphopeptide derived from human GSK3α around the phosphorylation site of Tyrosine 279. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of GSK3α/β (phospho-Y279/216) polyclonal antibody at 1:2000 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS(1×PBS,PH7.4) for 30min whole cell lysate Lane3:L02 treated with PBS(1×PBS,PH7.4) for 60min whole cell lysate Lane4:L02 treated with PBS(1×PBS,PH7.4) for 120min whole cell lysate Lane5:L02 treated with PBS(1×PBS,PH7.4) for 180min whole cell lysate Lane6:L02 treated with PBS(1×PBS,PH7.4) for 240min whole cell lysate Lane7:C6 whole cell lysate
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  Immunohistochemistry (IHC) analyzes of p-GSK3α/β (Y279/216) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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