Product Name :
IkB-α (phospho-S32/S36) polyclonal antibody Background :
Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation Serines 32 and 36. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ , and each appear to make essential contributions to IκB phosphorylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-IκB-α (S32+S36) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at Ser32+S36. Immunogen :
Synthetic phosphopeptide derived from human IkB-α around the phosphorylation site of Serine 32/36. Conjugate :
Unconjugated Modification :
Phosphorylation
IkB-α (phospho-S32/S36) polyclonal antibody Background :
Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation Serines 32 and 36. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ , and each appear to make essential contributions to IκB phosphorylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-IκB-α (S32+S36) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at Ser32+S36. Immunogen :
Synthetic phosphopeptide derived from human IkB-α around the phosphorylation site of Serine 32/36. Conjugate :
Unconjugated Modification :
Phosphorylation
-
Western blot (WB) analysis of p-IkB-α (S32/S36) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:3T3-L1 whole cell lysate(40ug)
-
Immunohistochemistry (IHC) analyzes of p-IkB-α (S32/S36) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Activation of PPARγ by a Natural Flavonoid Modulator, Apigenin Ameliorates Obesity-Related Inflammation Via Regulation of Macrophage Polarization
PMCID: Pubmed No.:27374313
The RCAN1 inhibits NF- κ B and suppresses lymphoma growth in mice
PMCID: Pubmed No.:26492364
Involvement of p120 in LPS-induced NF- B activation and IL-8 production in human bronchial epithelial cells
PMCID: Pubmed No.:20172018
Reactive Oxygen Species Released from Hypoxic Hepatocytes Regulates MMP-2 Expression in Hepatic Stellate Cells
PMCID: Pubmed No.:21731450
XJP-1, a novel ACEI, with anti-inflammatory properties in HUVECs
PMCID: Pubmed No.:21803355
Prevention of FGF-2-induced angiogenesis by scopoletin, a coumarin compound isolated from Erycibe obtusifolia Benth, and its mechanism of action
PMCID: Pubmed No.:21896339
Apple polysaccharides induce apoptosis in colorectal cancer cells.
PMCID: Pubmed No.:22470124
Norisoboldine inhibits the production of interleukin-6 in fibroblast-like synoviocytes from adjuvant arthritis rats through PKC/MAPK/NF-κB-p65/CREB pathways
PMCID: Pubmed No.:22473817
Suppression of endothelial cell adhesion by XJP-1, a new phenolic compound derived from banana peel
PMCID: Pubmed No.:22609942
Increased Inflammation and Brain Injury after Transient Focal Cerebral Ischemia in Activating Transcription Factor 3 Knockout Mice
PMCID: Pubmed No.:22704967
Chlorogenic acid reduces liver inflammation and fibrosis through inhibition of toll-like receptor 4 signaling pathway
PMCID: Pubmed No.:23146752
The effect of 2, 3, 4′, 5-tetrahydroxystilbene-2-0-β-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms
PMCID: Pubmed No.:23178522
Mindin is a critical mediator of ischemic brain injury in an experimental stroke model
PMCID: Pubmed No.:23360804
Tumor Necrosis Factor Receptor Associated Factor 5 is an Essential Mediator of Ischemic Brain Infarction
PMCID: Pubmed No.:23413803
Effect of chlorogenic acid on LPS-induced proinflammatory signaling in hepatic stellate cells
PMCID: Pubmed No.:23483217
Wogonin inhibits H2O2-induced angiogenesis via suppressing PI3K/Akt/NF-κB signaling pathway.
PMCID: Pubmed No.:24534483
Interferon Regulatory Factor 8 Protects Against Cerebral Ischaemic‐Reperfusion Injury
PMCID: Pubmed No.:24528256
MicroRNA-155 Is Involved in the Pathogenesis of Ulcerative Colitis by Targeting FOXO3a
PMCID: Pubmed No.:24583476
Wogonin inhibits LPS-induced tumor angiogenesis via suppressing PI3K/Akt/NF-κB signaling
PMCID: Pubmed No.:24858369
Dishevelled‐3 activates p65 to upregulate p120‐catenin transcription via a p38‐dependent pathway in non‐small cell lung cancer
PMCID: Pubmed No.:25156800
Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB)
PMCID: Pubmed No.:25284342
Interleukin-17 (IL-17)-induced MicroRNA 873 (miR-873) Contributes to the Pathogenesis of Experimental Autoimmune Encephalomyelitis by Targeting A20 Ubiquitin-editing Enzyme.
PMCID: Pubmed No.:25183005
LL202 inhibits lipopolysaccharide-induced angiogenesis in vivo and in vitro
PMCID: Pubmed No.:19428938
Interleukin-17 (IL-17)-induced MicroRNA 873 (miR-873) Contributes to the Pathogenesis of Experimental Autoimmune Encephalomyelitis by Targeting A20 Ubiquitin-editing Enzyme.
PMCID: Pubmed No.:25183005
Protective effects of resveratrol on postmenopausal osteoporosis: regulation of SIRT1-NF-κB signaling pathway
PMCID: Pubmed No.:25377437
Ginsenoside Rb1 attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of the JNK and p38 signaling pathways
PMCID: Pubmed No.:25912763
The protective effect of CDDO-Me on lipopolysaccharide-induced acute lung injury in mice.
PMCID: Pubmed No.:25614226
Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats.
PMCID: Pubmed No.:25655087
Protective effects of protocatechuic acid on acute lung injury induced by lipopolysaccharide in mice via p38MAPK and NF-κB signal pathways
PMCID: Pubmed No.:25841318
DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner
PMCID: Pubmed No.:26456500
Dishevelled‐3 activates p65 to upregulate p120‐catenin transcription via a p38‐dependent pathway in non‐small cell lung cancer
PMCID: Pubmed No.:25156800
Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB)
PMCID: Pubmed No.:25284342
Interleukin-17 (IL-17)-induced MicroRNA 873 (miR-873) Contributes to the Pathogenesis of Experimental Autoimmune Encephalomyelitis by Targeting A20 Ubiquitin-editing Enzyme.
PMCID: Pubmed No.:25183005
LL202 inhibits lipopolysaccharide-induced angiogenesis in vivo and in vitro
PMCID: Pubmed No.:19428938
Interleukin-17 (IL-17)-induced MicroRNA 873 (miR-873) Contributes to the Pathogenesis of Experimental Autoimmune Encephalomyelitis by Targeting A20 Ubiquitin-editing Enzyme.
PMCID: Pubmed No.:25183005
Protective effects of resveratrol on postmenopausal osteoporosis: regulation of SIRT1-NF-κB signaling pathway
PMCID: Pubmed No.:25377437
DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner
PMCID: Pubmed No.:26456500
Wogonin inhibits H2O2-induced angiogenesis via suppressing PI3K/Akt/NF-κB signaling pathway.
PMCID: Pubmed No.:24534483
Interferon Regulatory Factor 8 Protects Against Cerebral Ischaemic‐Reperfusion Injury
PMCID: Pubmed No.:24528256
MicroRNA-155 Is Involved in the Pathogenesis of Ulcerative Colitis by Targeting FOXO3a
PMCID: Pubmed No.:24583476
Alpha‐Lipoic Acid Promotes Osteoblastic Formation in H2O2‐Treated MC3T3‐E1 Cells and Prevents Bone Loss in Ovariectomized Rats
PMCID: Pubmed No.:25655087
The mAb against adipocyte fatty acid-binding protein 2E4 attenuates the inflammation in the mouse model of high-fat diet-induced obesity via toll-like receptor 4 pathway
PMCID: Pubmed No.:25596549
Anti-inflammatory capacity of Lactobacillus rhamnosus GG in monophasic variant Salmonella infected piglets is correlated with impeding NLRP6-mediated host inflammatory responses
PMCID: Pubmed No.:29103703
Enhanced IMP3 Expression Activates NF-кB Pathway and Promotes Renal Cell Carcinoma Progression
PMCID: Pubmed No.:25919292
Protective Effects of Pterostilbene on Lipopolysaccharide-Induced Acute Lung Injury in Mice by Inhibiting NF-κB and Activating Nrf2/HO-1 Signaling Pathways.
PMCID: Pubmed No.:33633565
Alpinetin prevents inflammatory responses in OVA-induced allergic asthma through modulating PI3K/AKT/NF-κB and HO-1 signaling pathways in mice
PMCID: Pubmed No.:33039967
Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation
PMCID: Pubmed No.:31572058
Evidence of anti-inflammatory activity of Schizandrin A in animal models of acute inflammation
PMCID: Pubmed No.:32076762
Streptolysin O derived from Streptococcus pyogenes inhibits RANKL‑induced osteoclastogenesis through the NF‑κB signaling pathway
PMCID: Pubmed No.:30431141
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.