MEK-4/ MAP2K4 (phospho-T261) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
MEK-4/ MAP2K4 (phospho-T261) polyclonal antibody Background :
The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-SEK1/MKK4 (T261) polyclonal antibody detects endogenous levels of SEK1/MKK4 protein only when phosphorylated at Thr261 Immunogen :
Synthetic phosphopeptide derived from human MEK-4 around the phosphorylation site of Threonine 261. Conjugate :
Unconjugated Modification :
Phosphorylation
MEK-4/ MAP2K4 (phospho-T261) polyclonal antibody Background :
The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-SEK1/MKK4 (T261) polyclonal antibody detects endogenous levels of SEK1/MKK4 protein only when phosphorylated at Thr261 Immunogen :
Synthetic phosphopeptide derived from human MEK-4 around the phosphorylation site of Threonine 261. Conjugate :
Unconjugated Modification :
Phosphorylation
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Immunohistochemistry (IHC) analyzes of p-MEK-4/ MAP2K4 (T261) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.