Stat3 (phospho-Y705) polyclonal antibody 
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Product Name :
Stat3 (phospho-Y705) polyclonal antibody Background :
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Stat3 (Y705) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705 Immunogen :
Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Tyrosine 705. Conjugate :
Unconjugated Modification :
Phosphorylation
Stat3 (phospho-Y705) polyclonal antibody Background :
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Stat3 (Y705) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705 Immunogen :
Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Tyrosine 705. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of Stat3 (phospho-Y705) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:Hela treated with IFN-γ(100ng/ml, 30min) whole cell lysate(40μg) Lane3:Jurkat whole cell lysate(40μg) Lane4:Jurkat treated with H2O2(100nM/ml, 30min) whole cell lysate(40μg) -
Immunohistochemistry (IHC) analyzes of p-STAT3 (Y705) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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CCL5 secreted by tumor associated macrophages may be a new target in treatment of gastric cancer
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Anti-inflammatory and immunomodulatory mechanisms of artemisinin on contact hypersensitivity
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Inhibition of the STAT3 signaling pathway is involved in the antitumor activity of cepharanthine in SaOS2 cells
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Norisoboldine alleviates joint destruction in rats with adjuvant-induced arthritis by reducing RANKL, IL-6, PGE2, and MMP-13 expression
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SH2B1β interacts with STAT3 and enhances fibroblast growth factor 1-induced gene expression during neuronal differentiation
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Hydroxy-safflor yellow A inhibits neuroinflammation mediated by Aβ1–42 in BV-2 cells
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LFG-500 Inhibits the Invasion of Cancer Cells via Down-Regulation of PI3K/AKT/NF-κB Signaling Pathway
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Bone marrow microenvironment confers imatinib resistance to chronic myelogenous leukemia and oroxylin A reverses the resistance by suppressing Stat3 pathway
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Diosmin protects against cerebral ischemia/reperfusion injury through activating JAK2/STAT3 signal pathway in mice
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Inhibition of IL-17A Suppresses Enhanced-Tumor Growth in Low Dose Pre-Irradiated Tumor Beds
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Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9
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CCL5 secreted by tumor associated macrophages may be a new target in treatment of gastric cancer
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microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia
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Inhibition of IL-17A Suppresses Enhanced-Tumor Growth in Low Dose Pre-Irradiated Tumor Beds
PMCID: Pubmed No.:25181290
Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9
PMCID: Pubmed No.:26359917
CCL5 secreted by tumor associated macrophages may be a new target in treatment of gastric cancer
PMCID: Pubmed No.:26796278
microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia
PMCID: Pubmed No.:27086927
SH2B1β interacts with STAT3 and enhances fibroblast growth factor 1-induced gene expression during neuronal differentiation
PMCID: Pubmed No.:24396070
Hydroxy-safflor yellow A inhibits neuroinflammation mediated by Aβ1–42 in BV-2 cells
PMCID: Pubmed No.:24412680
LFG-500 Inhibits the Invasion of Cancer Cells via Down-Regulation of PI3K/AKT/NF-κB Signaling Pathway
PMCID: Pubmed No.:24618693
Bone marrow microenvironment confers imatinib resistance to chronic myelogenous leukemia and oroxylin A reverses the resistance by suppressing Stat3 pathway
PMCID: Pubmed No.:24671465
Diosmin protects against cerebral ischemia/reperfusion injury through activating JAK2/STAT3 signal pathway in mice
PMCID: Pubmed No.:24680937
Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway
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Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9
PMCID: Pubmed No.:26359917
Promoting effects of IL‑23 on myocardial ischemia and reperfusion are associated with increased expression of IL‑17A and upregulation of the JAK2‑STAT3 signaling pathway
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Uterine glands coordinate on-time embryo implantation and impact endometrial decidualization for pregnancy success
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IL-9-triggered lncRNA Gm13568 regulates Notch1 in astrocytes through interaction with CBP/P300: contribute to the pathogenesis of experimental autoimmune encephalomyelitis
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MiR‐409‐3p and MiR‐1896 co‐operatively participate in IL‐17‐induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling
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PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization
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Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury
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Th9 cytokines curb cervical cancer progression and immune evasion
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LPS‑induced proinflammatory cytokine expression in human airway epithelial cells and macrophages via NF‑κB, STAT3 or AP‑1 activation
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Combination of RSK inhibitor LJH-685 and FLT3 inhibitor FF-10101 promoted apoptosis and proliferation inhibition of AML cell lines
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.