FAK (phospho-Y397) polyclonal antibody 
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Product Name :
FAK (phospho-Y397) polyclonal antibody Background :
Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FAK (phospho-Y397) polyclonal antibody detects endogenous levels of FAK protein only when phosphorylated at Tyr397. Immunogen :
Synthetic phosphopeptide derived from human FAK around the phosphorylation site of Serine 397. Conjugate :
Unconjugated Modification :
Phosphorylation
FAK (phospho-Y397) polyclonal antibody Background :
Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FAK (phospho-Y397) polyclonal antibody detects endogenous levels of FAK protein only when phosphorylated at Tyr397. Immunogen :
Synthetic phosphopeptide derived from human FAK around the phosphorylation site of Serine 397. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of FAK (phospho-Y397) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-FAK (Y397) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Modeled microgravity causes changes in the cytoskeleton and focal adhesions, and decreases in migration in malignant human MCF-7 cells
PMCID: Pubmed No.:19730978
Inhibition of FAK prevents blister formation in the neonatal mouse model of pemphigus vulgaris
PMCID: Pubmed No.:22320676
Gleditsioside B, a triterpene saponin isolated from the anomalous fruits of Gleditsia sinensis Lam., abrogates bFGF-induced endothelial cell migration through preventing the activation of MMP-2 and FAK via inhibiting ERK and PI3K/AKT signaling pathways
PMCID: Pubmed No.:23026290
Neural nitric oxide synthase participates in pemphigus vulgaris acantholysis through upregulation of Rous sarcoma, mammalian target of rapamycin and focal adhesion kinase
PMCID: Pubmed No.:23362871
Association of integrin beta1 and c-MET in mediating EGFR TKI gefitinib resistance in non-small cell lung cancer
PMCID: Pubmed No.:23402326
Cell-surface GRP78 facilitates colorectal cancer cell migration and invasion
PMCID: Pubmed No.:23485528
Activation of matrix metalloproteinase-26 by HOXA10 promotes embryo adhesion in vitro.
PMCID: Pubmed No.:24565841
Plasma miR-185 is decreased in esophageal squamous cell carcinoma patients and might suppress tumor migration and invasion by targeting RAGE.
PMCID: Pubmed No.:26316588
Pharmacological Inhibition of Focal Adhesion Kinase Attenuates Cardiac Fibrosis in Mice Cardiac Fibroblast and Post-Myocardial-Infarction Models
PMCID: Pubmed No.:26330161
Activation of matrix metalloproteinase-26 by HOXA10 promotes embryo adhesion in vitro.
PMCID: Pubmed No.:24565841
Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-
PMCID: Pubmed No.:25990368
Herbal compound Teng‑Long‑Bu‑Zhong‑Tang inhibits metastasis in human RKO colon carcinoma
PMCID: Pubmed No.:29344222
Sulfatide interacts with and activates integrin αVβ3 in human hepatocellular carcinoma cells
PMCID: Pubmed No.:27145276
Twist promotes reprogramming of glucose metabolism in breast cancer cells through PI3K/AKT and p53 signaling pathways
PMCID: Pubmed No.:26342198
Tumor-secreted GRP78 facilitates the migration of macrophages into tumors by promoting cytoskeleton remodeling
PMCID: Pubmed No.:30959099
Croton tiglium essential oil compounds have anti-proliferative and pro-apoptotic effects in A549 lung cancer cell lines
PMCID: Pubmed No.:32357169
GSNOR deficiency promotes tumor growth via FAK1 S-nitrosylation
PMCID: Pubmed No.:36656716
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.