Product Name :
mTOR (phospho-S2448) polyclonal antibody Background :
mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-mTOR (S2448) polyclonal antibody detects endogenous levels of mTOR protein when phosphorylated at Ser2448. Immunogen :
Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2442. Conjugate :
Unconjugated Modification :
Phosphorylation
mTOR (phospho-S2448) polyclonal antibody Background :
mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-mTOR (S2448) polyclonal antibody detects endogenous levels of mTOR protein when phosphorylated at Ser2448. Immunogen :
Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2442. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-mTOR (S2448) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with insulin(100nM,30mins) Lane2:Raw264.7 cell lysate treated with insulin(100nM,30mins) Lane3:PC12 cell lysate treated with insulin(100nM,30mins)
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Immunohistochemistry (IHC) analyzes of p-mTOR (S2448) pAb in paraffin-embedded human brain tissue.
Inhibition of PI3K/AKT/mTOR signaling pathway promotes autophagy of articular chondrocytes and attenuates inflammatory response in rats with osteoarthritis
PMCID: Pubmed No.:28320092
Curcumin attenuates lipopolysaccharide/d-galactosamine-induced acute liver injury by activating Nrf2 nuclear translocation and inhibiting NF-kB activation
PMCID: Pubmed No.:28448872
Knockdown of TNF receptor-associated factor 2 (TRAF2) modulates in vitro growth of TRAIL-treated prostate cancer cells
PMCID: Pubmed No.:28667915
Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway
PMCID: Pubmed No.:28714804
ROS-activated p38 MAPK/ERK-Akt cascade plays a central role in palmitic acid-stimulated hepatocyte proliferation.
PMCID: Pubmed No.:21620957
Cucurmosin induces apoptosis of BxPC-3 human pancreatic cancer cells via inactivation of the EGFR signaling pathway.
PMCID: Pubmed No.:22139427
E Platinum, a newly synthesized platinum compound, induces autophagy via inhibiting phosphorylation of mTOR in gastric carcinoma BGC-823 cells
PMCID: Pubmed No.:22322152
Disruption of mindin exacerbates cardiac hypertrophy and fibrosis
PMCID: Pubmed No.:22367478
Beclin 1-mediated autophagy in hepatocellular carcinoma cells: Implication in anticancer efficiency of oroxylin A via inhibition of mTOR signaling
PMCID: Pubmed No.:22560876
Estrogen-Sensitive PTPRO Expression Represses Hepatocellular Carcinoma Progression by Control of STAT3
PMCID: Pubmed No.:22821478
Wogonoside induces autophagy in MDA-MB-231 cells by regulating MAPK-mTOR pathway
PMCID: Pubmed No.:23000445
ZFX regulates glioma cell proliferation and survival in vitro and in vivo
PMCID: Pubmed No.:23322077
miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6.
PMCID: Pubmed No.:23624080
Cucurmosin induces the apoptosis of human pancreatic cancer CFPAC-1 cells by inactivating the PDGFR-b signalling pathway
PMCID: Pubmed No.:23950591
Peroxisome proliferator-activated receptor-γ interrupts angiogenic signal transduction via transrepression of platelet-derived growth factor-β receptor in hepatic stellate cells
PMCID: Pubmed No.:24259663
Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway
PMCID: Pubmed No.:24366110
Luteolin is effective in the non‐small‐cell lung cancer model with L858R/T790M EGFR mutation and erlotinib resistance
PMCID: Pubmed No.:24471765
Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions
PMCID: Pubmed No.:24504086
Rapamycin attenuates visible light-induced injury in retinal photoreceptor cells via inhibiting endoplasmic reticulum stress
PMCID: Pubmed No.:24607296
Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost,< i> Cyprinus carpio
PMCID: Pubmed No.:24780358
Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells
PMCID: Pubmed No.:24779927
Berberine inhibits LPS-induced TF procoagulant activity and expression through NF-κB/p65, Akt and MAPK pathway in THP-1 cells
PMCID: Pubmed No.:24905527
Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells
PMCID: Pubmed No.:25193113
Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway
PMCID: Pubmed No.:25213258
Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway
PMCID: Pubmed No.:25564356
Effect of baicalin-copper on the induction of apoptosis in human hepatoblastoma cancer HepG2 cells
PMCID: Pubmed No.:25694047
Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway
PMCID: Pubmed No.:26318432
Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest
PMCID: Pubmed No.:27035647
Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells
PMCID: Pubmed No.:25193113
Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway
PMCID: Pubmed No.:25213258
Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway
PMCID: Pubmed No.:25564356
Peroxisome proliferator-activated receptor-γ interrupts angiogenic signal transduction via transrepression of platelet-derived growth factor-β receptor in hepatic stellate cells
PMCID: Pubmed No.:24259663
Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest
PMCID: Pubmed No.:27035647
Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost,< i> Cyprinus carpio
PMCID: Pubmed No.:24780358
Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells
PMCID: Pubmed No.:24779927
Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway
PMCID: Pubmed No.:24366110
Luteolin is effective in the non‐small‐cell lung cancer model with L858R/T790M EGFR mutation and erlotinib resistance
PMCID: Pubmed No.:24471765
Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions
PMCID: Pubmed No.:24504086
Rapamycin attenuates visible light-induced injury in retinal photoreceptor cells via inhibiting endoplasmic reticulum stress
PMCID: Pubmed No.:24607296
Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways
PMCID: Pubmed No.:27576342
Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation
PMCID: Pubmed No.:25751394
Sirt1 decreased adipose inflammation by interacting with Akt2 and inhibiting mTOR/S6K1 pathway in mice
PMCID: Pubmed No.:27317762
LINGO-1 antibody ameliorates myelin impairment and spatial memory deficits in experimental autoimmune encephalomyelitis mice
PMCID: Pubmed No.:26383267
SPOCK1 promotes the growth of Osteosarcoma cells through mTOR-S6K signaling pathway
PMCID: Pubmed No.:28869894
Effect of baicalin-copper on the induction of apoptosis in human hepatoblastoma cancer HepG2 cells
PMCID: Pubmed No.:25694047
l-Theanine attenuates cadmium-induced neurotoxicity through the inhibition of oxidative damage and tau hyperphosphorylation
PMCID: Pubmed No.:27649883
Carnosic Acid Prevents Beta-Amyloid-Induced Injury in Human Neuroblastoma SH-SY5Y Cells via the Induction of Autophagy
PMCID: Pubmed No.:27168327
Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway
PMCID: Pubmed No.:25564356
Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest
PMCID: Pubmed No.:27035647
Bergenin suppresses the growth of colorectal cancer cells by inhibiting PI3K/AKT/mTOR signaling pathway
PMCID: Pubmed No.:37259388
Arctigenin functions as a selective agonist of estrogen receptor β to restrict mTORC1 activation and consequent Th17 differentiation
PMCID: Pubmed No.:27863380
PKG II reverses HGF-triggered cellular activities by phosphorylating serine 985 of c-Met in gastric cancer cells
PMCID: Pubmed No.:27147579
Bergenin impedes the generation of extracellular matrix in glomerular mesangial cells and ameliorates diabetic nephropathy in mice by inhibiting oxidative stress via the mTOR/β-TrcP/Nrf2 pathway
PMCID: Pubmed No.:31494242
Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity
PMCID: Pubmed No.:31849465
Concurrence of autophagy with apoptosis in alveolar epithelial cells contributes to chronic pulmonary toxicity induced by methamphetamine
PMCID: Pubmed No.:29956395
Citrate activates autophagic death of prostate cancer cells via downregulation CaMKII/AKT/mTOR pathway
PMCID: Pubmed No.:33744326
mTOR‐autophagy promotes pulmonary senescence through IMP1 in chronic toxicity of methamphetamine
PMCID: Pubmed No.:32918374
The Akt pathway mediates the protective effects of myeloid differentiation protein 1 in pathological cardiac remodelling
PMCID: Pubmed No.:34041859
Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo
PMCID: Pubmed No.:34648903
SIRT3 Protects Rotenone-induced Injury in SH-SY5Y Cells by Promoting Autophagy through the LKB1-AMPK-mTOR Pathway
PMCID: Pubmed No.:29896416
Alogliptin improves endothelial function by promoting autophagy in perivascular adipose tissue of obese mice through a GLP-1-dependent mechanism
PMCID: Pubmed No.:30447331
Neuregulin 4 alleviates hepatic steatosis via activating AMPK/mTOR-mediated autophagy in aged mice fed a high fat diet
PMCID: Pubmed No.:32726654
Astragaloside IV alleviates liver injury in type 2 diabetes due to promotion of AMPK/mTOR‑mediated autophagy
PMCID: Pubmed No.:33846768
A novel pathogenic splice site variation in STK11 gene results in Peutz–Jeghers syndrome
PMCID: Pubmed No.:34080793
Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway
PMCID: Pubmed No.:30066869
Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia
PMCID: Pubmed No.:29675372
Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia
PMCID: Pubmed No.:29675372
Methyl Helicterate Inhibits Hepatic Stellate Cell Activation Through Modulation of Apoptosis and Autophagy
PMCID: Pubmed No.:30466104
Functional Characterization of FH Mutation c.557G>A Underlies Uterine Leiomyomas
PMCID: Pubmed No.:35163394
Increased homocysteine regulated by androgen activates autophagy by suppressing the mammalian target of rapamycin pathway in the granulosa cells of polycystic ovary syndrome mice
PMCID: Pubmed No.:35485387
Homoplantaginin attenuates high glucose-induced vascular endothelial cell apoptosis through promoting autophagy via the AMPK/TFEB pathway
PMCID: Pubmed No.:36879478
Sweroside alleviates hepatic steatosis in part by activating AMPK/mTOR-mediated autophagy in mice
PMCID: Pubmed No.:37269482
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.