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mTOR (phospho-S2448) polyclonal antibody BS4706
  • Western blot (WB) analysis of p-mTOR (S2448) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with insulin(100nM,30mins) Lane2:Raw264.7 cell lysate treated with insulin(100nM,30mins) Lane3:PC12 cell lysate treated with insulin(100nM,30mins)
  • Immunohistochemistry (IHC) analyzes of p-mTOR (S2448) pAb in paraffin-embedded human brain tissue.
Product NamemTOR (phospho-S2448) polyclonal antibody
Catalog No.BS4706
Swiss-ProtP42345
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000_x000D_ IHC: 1:50~1:200
BiowMW~ 289 kDa
Alternative NameSerine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylation
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50ul $168
100ul $298
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Product Name :
mTOR (phospho-S2448) polyclonal antibody
Background :
mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p-mTOR (S2448) polyclonal antibody detects endogenous levels of mTOR protein when phosphorylated at Ser2448.
Immunogen :
Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2442.
Conjugate :
Unconjugated
Modification :
Phosphorylation
  • Western blot (WB) analysis of p-mTOR (S2448) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with insulin(100nM,30mins) Lane2:Raw264.7 cell lysate treated with insulin(100nM,30mins) Lane3:PC12 cell lysate treated with insulin(100nM,30mins)
  • Immunohistochemistry (IHC) analyzes of p-mTOR (S2448) pAb in paraffin-embedded human brain tissue.
Inhibition of PI3K/AKT/mTOR signaling pathway promotes autophagy of articular chondrocytes and attenuates inflammatory response in rats with osteoarthritis PMCID:    Pubmed No.:28320092 Curcumin attenuates lipopolysaccharide/d-galactosamine-induced acute liver injury by activating Nrf2 nuclear translocation and inhibiting NF-kB activation PMCID:    Pubmed No.:28448872 Knockdown of TNF receptor-associated factor 2 (TRAF2) modulates in vitro growth of TRAIL-treated prostate cancer cells PMCID:    Pubmed No.:28667915 Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway PMCID:    Pubmed No.:28714804 ROS-activated p38 MAPK/ERK-Akt cascade plays a central role in palmitic acid-stimulated hepatocyte proliferation. PMCID:    Pubmed No.:21620957 Cucurmosin induces apoptosis of BxPC-3 human pancreatic cancer cells via inactivation of the EGFR signaling pathway. PMCID:    Pubmed No.:22139427 E Platinum, a newly synthesized platinum compound, induces autophagy via inhibiting phosphorylation of mTOR in gastric carcinoma BGC-823 cells PMCID:    Pubmed No.:22322152 Disruption of mindin exacerbates cardiac hypertrophy and fibrosis PMCID:    Pubmed No.:22367478 Beclin 1-mediated autophagy in hepatocellular carcinoma cells: Implication in anticancer efficiency of oroxylin A via inhibition of mTOR signaling PMCID:    Pubmed No.:22560876 Estrogen-Sensitive PTPRO Expression Represses Hepatocellular Carcinoma Progression by Control of STAT3 PMCID:    Pubmed No.:22821478 Wogonoside induces autophagy in MDA-MB-231 cells by regulating MAPK-mTOR pathway PMCID:    Pubmed No.:23000445 ZFX regulates glioma cell proliferation and survival in vitro and in vivo PMCID:    Pubmed No.:23322077 miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6. PMCID:    Pubmed No.:23624080 Cucurmosin induces the apoptosis of human pancreatic cancer CFPAC-1 cells by inactivating the PDGFR-b signalling pathway PMCID:    Pubmed No.:23950591 Peroxisome proliferator-activated receptor-γ interrupts angiogenic signal transduction via transrepression of platelet-derived growth factor-β receptor in hepatic stellate cells PMCID:    Pubmed No.:24259663 Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway PMCID:    Pubmed No.:24366110 Luteolin is effective in the non‐small‐cell lung cancer model with L858R/T790M EGFR mutation and erlotinib resistance PMCID:    Pubmed No.:24471765 Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions PMCID:    Pubmed No.:24504086 Rapamycin attenuates visible light-induced injury in retinal photoreceptor cells via inhibiting endoplasmic reticulum stress PMCID:    Pubmed No.:24607296 Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost,< i> Cyprinus carpio PMCID:    Pubmed No.:24780358 Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells PMCID:    Pubmed No.:24779927 Berberine inhibits LPS-induced TF procoagulant activity and expression through NF-κB/p65, Akt and MAPK pathway in THP-1 cells PMCID:    Pubmed No.:24905527 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway PMCID:    Pubmed No.:25213258 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Effect of baicalin-copper on the induction of apoptosis in human hepatoblastoma cancer HepG2 cells PMCID:    Pubmed No.:25694047 Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway PMCID:    Pubmed No.:26318432 Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest PMCID:    Pubmed No.:27035647 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway PMCID:    Pubmed No.:25213258 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Peroxisome proliferator-activated receptor-γ interrupts angiogenic signal transduction via transrepression of platelet-derived growth factor-β receptor in hepatic stellate cells PMCID:    Pubmed No.:24259663 Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest PMCID:    Pubmed No.:27035647 Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost,< i> Cyprinus carpio PMCID:    Pubmed No.:24780358 Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells PMCID:    Pubmed No.:24779927 Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway PMCID:    Pubmed No.:24366110 Luteolin is effective in the non‐small‐cell lung cancer model with L858R/T790M EGFR mutation and erlotinib resistance PMCID:    Pubmed No.:24471765 Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions PMCID:    Pubmed No.:24504086 Rapamycin attenuates visible light-induced injury in retinal photoreceptor cells via inhibiting endoplasmic reticulum stress PMCID:    Pubmed No.:24607296 Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways PMCID:    Pubmed No.:27576342 Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation PMCID:    Pubmed No.:25751394 Sirt1 decreased adipose inflammation by interacting with Akt2 and inhibiting mTOR/S6K1 pathway in mice PMCID:    Pubmed No.:27317762 LINGO-1 antibody ameliorates myelin impairment and spatial memory deficits in experimental autoimmune encephalomyelitis mice PMCID:    Pubmed No.:26383267 SPOCK1 promotes the growth of Osteosarcoma cells through mTOR-S6K signaling pathway PMCID:    Pubmed No.:28869894 Effect of baicalin-copper on the induction of apoptosis in human hepatoblastoma cancer HepG2 cells PMCID:    Pubmed No.:25694047 l-Theanine attenuates cadmium-induced neurotoxicity through the inhibition of oxidative damage and tau hyperphosphorylation PMCID:    Pubmed No.:27649883 Carnosic Acid Prevents Beta-Amyloid-Induced Injury in Human Neuroblastoma SH-SY5Y Cells via the Induction of Autophagy PMCID:    Pubmed No.:27168327 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest PMCID:    Pubmed No.:27035647 Bergenin suppresses the growth of colorectal cancer cells by inhibiting PI3K/AKT/mTOR signaling pathway PMCID:    Pubmed No.:37259388 Arctigenin functions as a selective agonist of estrogen receptor β to restrict mTORC1 activation and consequent Th17 differentiation PMCID:    Pubmed No.:27863380 PKG II reverses HGF-triggered cellular activities by phosphorylating serine 985 of c-Met in gastric cancer cells PMCID:    Pubmed No.:27147579 Bergenin impedes the generation of extracellular matrix in glomerular mesangial cells and ameliorates diabetic nephropathy in mice by inhibiting oxidative stress via the mTOR/β-TrcP/Nrf2 pathway PMCID:    Pubmed No.:31494242 Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity PMCID:    Pubmed No.:31849465 Concurrence of autophagy with apoptosis in alveolar epithelial cells contributes to chronic pulmonary toxicity induced by methamphetamine PMCID:    Pubmed No.:29956395 Citrate activates autophagic death of prostate cancer cells via downregulation CaMKII/AKT/mTOR pathway PMCID:    Pubmed No.:33744326 mTOR‐autophagy promotes pulmonary senescence through IMP1 in chronic toxicity of methamphetamine PMCID:    Pubmed No.:32918374 The Akt pathway mediates the protective effects of myeloid differentiation protein 1 in pathological cardiac remodelling PMCID:    Pubmed No.:34041859 Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo PMCID:    Pubmed No.:34648903 SIRT3 Protects Rotenone-induced Injury in SH-SY5Y Cells by Promoting Autophagy through the LKB1-AMPK-mTOR Pathway PMCID:    Pubmed No.:29896416 Alogliptin improves endothelial function by promoting autophagy in perivascular adipose tissue of obese mice through a GLP-1-dependent mechanism PMCID:    Pubmed No.:30447331 Neuregulin 4 alleviates hepatic steatosis via activating AMPK/mTOR-mediated autophagy in aged mice fed a high fat diet PMCID:    Pubmed No.:32726654 Astragaloside IV alleviates liver injury in type 2 diabetes due to promotion of AMPK/mTOR‑mediated autophagy PMCID:    Pubmed No.:33846768 A novel pathogenic splice site variation in STK11 gene results in Peutz–Jeghers syndrome PMCID:    Pubmed No.:34080793 Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway PMCID:    Pubmed No.:30066869 Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia PMCID:    Pubmed No.:29675372 Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia PMCID:    Pubmed No.:29675372 Methyl Helicterate Inhibits Hepatic Stellate Cell Activation Through Modulation of Apoptosis and Autophagy PMCID:    Pubmed No.:30466104 Functional Characterization of FH Mutation c.557G>A Underlies Uterine Leiomyomas PMCID:    Pubmed No.:35163394 Increased homocysteine regulated by androgen activates autophagy by suppressing the mammalian target of rapamycin pathway in the granulosa cells of polycystic ovary syndrome mice PMCID:    Pubmed No.:35485387 Homoplantaginin attenuates high glucose-induced vascular endothelial cell apoptosis through promoting autophagy via the AMPK/TFEB pathway PMCID:    Pubmed No.:36879478 Sweroside alleviates hepatic steatosis in part by activating AMPK/mTOR-mediated autophagy in mice PMCID:    Pubmed No.:37269482
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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