Cofilin (phospho-S3) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Cofilin (phospho-S3) polyclonal antibody Background :
Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Cofilin (S3) polyclonal antibody detects endogenous levels of Cofilin protein only when phosphorylated at Ser3. Immunogen :
Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3. Conjugate :
Unconjugated Modification :
Phosphorylation
Cofilin (phospho-S3) polyclonal antibody Background :
Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Cofilin (S3) polyclonal antibody detects endogenous levels of Cofilin protein only when phosphorylated at Ser3. Immunogen :
Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3. Conjugate :
Unconjugated Modification :
Phosphorylation
-
Western blot (WB) analysis of p-Cofilin (S3) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-Cofilin (S3) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Madecassoside suppresses migration of fibroblasts from keloids: involvement of p38 kinase and PI3K signaling pathways
PMCID: Pubmed No.:22360962
Paeoniflorin Attenuates Lipopolysaccharide-Induced Permeability of Endothelial Cells: Involvements of F-Actin Expression and Phosphorylations of PI3K/Akt and PKC
PMCID: Pubmed No.:23053726
Effect of Robo4 on Retinal Endothelial Permeability
PMCID: Pubmed No.:23163536
Oxymatrine targets EGFRp-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells
PMCID: Pubmed No.:25527205
Oxymatrine targets EGFRp-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells
PMCID: Pubmed No.:25527205
Oxymatrine targets EGFRp-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells
PMCID: Pubmed No.:25527205
LIMK2 is required for membrane cytoskeleton reorganization of contracting airway smooth muscle
PMCID: Pubmed No.:34353741
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.