MEK1/2 (phospho-S218/222) polyclonal antibody 
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Product Name :
MEK1/2 (phospho-S218/222) polyclonal antibody Background :
MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-MEK1/2 (S218/222) polyclonal antibody detects endogenous levels of MEK-1 protein only when phosphorylated at Ser218. This antibody also detects endogenous levels of MEK-2 protein only when phosphorylated at Thr222. Immunogen :
Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 218. Conjugate :
Unconjugated Modification :
Phosphorylation
MEK1/2 (phospho-S218/222) polyclonal antibody Background :
MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-MEK1/2 (S218/222) polyclonal antibody detects endogenous levels of MEK-1 protein only when phosphorylated at Ser218. This antibody also detects endogenous levels of MEK-2 protein only when phosphorylated at Thr222. Immunogen :
Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 218. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-MEK1/2 (S218/222) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate treated with PMA(100nM,30mins) Lane2:Raw264.7 cell lysate treated with PMA(100nM,30mins) Lane3:NIH-3T3 cell lysate treated with PMA(100nM,30mins) Lane4: PC12 cell lysate treated with PMA(100nM,30mins) -
Immunohistochemistry (IHC) analyzes of p-MEK1/2 (S218/222) pAb in paraffin-embedded human breast carcinoma tissue.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.