4E-BP1 (phospho-T46) polyclonal antibody 
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MSDS
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Product Name :
4E-BP1 (phospho-T46) polyclonal antibody Background :
The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-4E-BP1 (T46) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr46. Immunogen :
Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Threonine 46. Conjugate :
Unconjugated Modification :
Phosphorylation
4E-BP1 (phospho-T46) polyclonal antibody Background :
The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-4E-BP1 (T46) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr46. Immunogen :
Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Threonine 46. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-4E-BP1 (T46) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:EC9706 whole cell lysate(40ug) Lane5:Panc1 whole cell lysate(40ug) Lane6:3T3-L1 whole cell lysate(40ug) Lane7:PC12 whole cell lysate(20ug) -
Immunohistochemistry (IHC) analyzes of p-4E-BP1 (T46) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of p-4E-BP1 (T46) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.