Product Name :
Histone H2A.X (phospho-S139) polyclonal antibody Background :
H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Histone H2A.X (S139) polyclonal antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139. Immunogen :
Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139. Conjugate :
Unconjugated Modification :
Phosphorylation
Histone H2A.X (phospho-S139) polyclonal antibody Background :
H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Histone H2A.X (S139) polyclonal antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139. Immunogen :
Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-Histone H2A.X (S139) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with colchicine(0.2µg/ML,24h) Lane2:sp2/0 cell lysate treated with colchicine(0.2µg/ML,24h) Lane3:PC12 cell lysate treated with colchicine(0.2µg/ML,24h)
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Immunohistochemistry (IHC) analyzes of p-Histone H2A.X (S139) pAb in paraffin-embedded human brain tissue.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.