Product Name :
NOS3 (phospho-S1177) polyclonal antibody Background :
NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-NOS3 (S1177) polyclonal antibody detects endogenous levels of NOS3 protein when phosphorylated at Ser1177. Immunogen :
Synthetic phosphopeptide derived from human NOS3 around the phosphorylation site of Serine 1177. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of p-NOS3 (S1177) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
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  Western blot (WB) analysis of p-NOS3 (S1177) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with PMA (60ng/ml, 5min) whole cell lysate(40ug) Lane3:HEK293T treated with PMA (60ng/ml, 15min) whole cell lysate(40ug) Lane4:HEK293T treated with PMA (60ng/ml, 30min) whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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