FoxO3A (phospho-S253) polyclonal antibody 
Datasheet
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Product Name :
FoxO3A (phospho-S253) polyclonal antibody Background :
FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-FoxO3A (S253) polyclonal antibody detects endogenous levels of FoxO3A protein when phosphorylated at Ser253. Immunogen :
Synthetic phosphopeptide derived from human FOXO3A around the phosphorylation site of Serine 253. Conjugate :
Unconjugated Modification :
Phosphorylation
FoxO3A (phospho-S253) polyclonal antibody Background :
FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-FoxO3A (S253) polyclonal antibody detects endogenous levels of FoxO3A protein when phosphorylated at Ser253. Immunogen :
Synthetic phosphopeptide derived from human FOXO3A around the phosphorylation site of Serine 253. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-FoxO3A (S253) pAb at 1:500 dilution Lane1:The Testis tissue lysate of Rat(40ug) Lane2:The Testis tissue lysate of Mouse(20ug) Lane3:Hela whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p-FOXO3A (S253) pAb in paraffin-embedded human breast carcinoma tissue.
Cellular FLICE-inhibitory protein protects against cardiac remodelling after myocardial infarction
PMCID: Pubmed No.:22202974
MicroRNA-224 promotes cell proliferation and tumor growth in human colorectal cancer by repressing PHLPP1 and PHLPP2
PMCID: Pubmed No.:23846336
MicroRNA-155 Is Involved in the Pathogenesis of Ulcerative Colitis by Targeting FOXO3a
PMCID: Pubmed No.:24583476
HDAC2 Selectively Regulates FOXO3a-Mediated Gene Transcription during Oxidative Stress-Induced Neuronal Cell Death.
PMCID: Pubmed No.:25609639
Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mice pre-adipocyte
PMCID: Pubmed No.:26113535
MicroRNA-155 Is Involved in the Pathogenesis of Ulcerative Colitis by Targeting FOXO3a
PMCID: Pubmed No.:24583476
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.