Caspase-1 p10 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Caspase-1 p10 polyclonal antibody Background :
Caspase-1 (interleukin-1 beta convertase) belongs to the Interleukin-1-beta converting enzyme subfamily of caspases which mediate many features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. Caspase-1 promotes maturation of interleukin IL-1 beta and interleukin 18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflammatory cytokines. Cleavage of Caspase-1 into the p20 and p10 subunits directly correlates with the progression of apoptosis in the cell resulting in the initiation of the caspase cascade. Proteolytic cleavage of the precursor caspase-1 at glycine residue 317 in human and 315 in mouse generates the functional caspase-1 subunits, known as p20 and p10 subunits. At the amino acid level, the mouse p10 subunit is 81% identical to the human p10 subunit. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase-1 p10 polyclonal antibody detects endogenous levels of Caspase 1 p10 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 327-372 of Human Caspase 1. Conjugate :
Unconjugated Modification :
Unmodification
Caspase-1 p10 polyclonal antibody Background :
Caspase-1 (interleukin-1 beta convertase) belongs to the Interleukin-1-beta converting enzyme subfamily of caspases which mediate many features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. Caspase-1 promotes maturation of interleukin IL-1 beta and interleukin 18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflammatory cytokines. Cleavage of Caspase-1 into the p20 and p10 subunits directly correlates with the progression of apoptosis in the cell resulting in the initiation of the caspase cascade. Proteolytic cleavage of the precursor caspase-1 at glycine residue 317 in human and 315 in mouse generates the functional caspase-1 subunits, known as p20 and p10 subunits. At the amino acid level, the mouse p10 subunit is 81% identical to the human p10 subunit. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase-1 p10 polyclonal antibody detects endogenous levels of Caspase 1 p10 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 327-372 of Human Caspase 1. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Caspase-1 p10 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse spleen tissue lysate Lane3:Rat spleen tissue lysate
Chronic glucocorticoid exposure activates BK-NLRP3 signal involving in hippocampal neuron damage
PMCID: Pubmed No.:28732502
Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro
PMCID: Pubmed No.:28605710
Sarsasapogenin ameliorates diabetes‐associated memory impairment and neuroinflammation through down‐regulation of PAR‐1 receptor
PMCID: Pubmed No.:33885189
Propofol Attenuates Inflammatory Damage via Inhibiting NLRP1-Casp1-Casp6 Signaling in Ischemic Brain Injury
PMCID: Pubmed No.:32999158
Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation
PMCID: Pubmed No.:36271349
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.