GPR105 polyclonal antibody 
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Product Name :
GPR105 polyclonal antibody Background :
G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR105, also designated P2Y14, is widely expressed throughout many brain regions where it localizes to glial cells, and specifically co-localizes with astrocytes. GPR105 is upregulated when a tissue is immunologically challenged with lipopolysaccharide, leading to the theory that GPR105 may play an important role in modulating peripheral and neuroimmune function. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GPR105 polyclonal antibody detects endogenous levels of GPR105 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 197-242 of Human GPR105. Conjugate :
Unconjugated Modification :
Unmodification
GPR105 polyclonal antibody Background :
G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR105, also designated P2Y14, is widely expressed throughout many brain regions where it localizes to glial cells, and specifically co-localizes with astrocytes. GPR105 is upregulated when a tissue is immunologically challenged with lipopolysaccharide, leading to the theory that GPR105 may play an important role in modulating peripheral and neuroimmune function. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GPR105 polyclonal antibody detects endogenous levels of GPR105 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 197-242 of Human GPR105. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of GPR105 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat kidney tissue lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.