ARHGEF2 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ARHGEF2 polyclonal antibody Background :
GEFs catalyze the release of GDP from inactive Ras proteins to ena-ble GTP binding. Lfc (also designated Rho/Rac guanine nucleotide exchange factor 2, lymphoid blast crisis-like 1, ARHGEF2, GEF-H1, KIAA0651 or LFP40) is an 894 amino acid GEF for Rho A, a member of the Ras family that regulates the Actin cytoskeleton during the formation of stress fibers. Lfc directly interacts with Cingulin, a junctional adapter that inhibits Rho A, thus preventing phorbol-12-myristate-13-acetate-induced contraction and apoptosis. Mutations in the p53 gene transcriptionally activate Lfc, thereby increasing tumor cell proliferation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ARHGEF2 polyclonal antibody detects endogenous levels of ARHGEF2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 425-470 of Human ARHGEF2. Conjugate :
Unconjugated Modification :
Unmodification
ARHGEF2 polyclonal antibody Background :
GEFs catalyze the release of GDP from inactive Ras proteins to ena-ble GTP binding. Lfc (also designated Rho/Rac guanine nucleotide exchange factor 2, lymphoid blast crisis-like 1, ARHGEF2, GEF-H1, KIAA0651 or LFP40) is an 894 amino acid GEF for Rho A, a member of the Ras family that regulates the Actin cytoskeleton during the formation of stress fibers. Lfc directly interacts with Cingulin, a junctional adapter that inhibits Rho A, thus preventing phorbol-12-myristate-13-acetate-induced contraction and apoptosis. Mutations in the p53 gene transcriptionally activate Lfc, thereby increasing tumor cell proliferation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ARHGEF2 polyclonal antibody detects endogenous levels of ARHGEF2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 425-470 of Human ARHGEF2. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of ARHGEF2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.