Product Name :
PCNA polyclonal antibody Background :
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PCNA polyclonal antibody detects endogenous levels of PCNA protein. Immunogen :
Synthetic peptide, corresponding to amino acids 98-143 of Human PCNA. Conjugate :
Unconjugated Modification :
Unmodification
PCNA polyclonal antibody Background :
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PCNA polyclonal antibody detects endogenous levels of PCNA protein. Immunogen :
Synthetic peptide, corresponding to amino acids 98-143 of Human PCNA. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of PCNA polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
-
Immunohistochemistry (IHC) analyzes of PCNA pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
-
Immunohistochemistry (IHC) analyzes of PCNA pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
PMCID: Pubmed No.:27039827
An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation
PMCID: Pubmed No.:24469720
Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway
PMCID: Pubmed No.:24824968
The effect of hyperbaric oxygen on intracephalic angiogenesis in rats with intracerebral hemorrhage
PMCID: Pubmed No.:24836574
PKM2 depletion induces the compensation of glutaminolysis through β-catenin/c-Myc pathway in tumor cells
PMCID: Pubmed No.:25041845
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
PMCID: Pubmed No.:27039827
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
PMCID: Pubmed No.:27039827
Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway
PMCID: Pubmed No.:24824968
An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation
PMCID: Pubmed No.:24469720
Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice
PMCID: Pubmed No.:28266555
mTORC1 regulates apoptosis and cell proliferation in pterygium via targeting autophagy and FGFR3
PMCID: Pubmed No.:28779179
Tumor-Educated Neutrophils Activate Mesenchymal Stem Cells to Promote Gastric Cancer Growth and Metastasis
PMCID: Pubmed No.:32903528
MiR-3571 modulates the proliferation and migration of vascular smooth muscle cells by targeting claudin 1
PMCID: Pubmed No.:35370461
BMP6 Promotes the Secretion of 17 Beta-Estradiol and Progesterone in Goat Ovarian Granulosa Cells
PMCID: Pubmed No.:36009721
Hesperidin Reversed Long-Term N-methyl-N-nitro-N-Nitroguanidine Exposure Induced EMT and Cell Proliferation by Activating Autophagy in Gastric Tissues of Rats
PMCID: Pubmed No.:36558440
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.