AXIN1 polyclonal antibody 
Datasheet
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Product Name :
AXIN1 polyclonal antibody Background :
β-catenin is a component of both the cadherin cell adhesion system and the Wnt signaling pathway. Wnt signaling increases the amount of β-catenin by preventing its ubiquitination and degradation, allowing its direct interaction with transcription factors of the lymphoid enhancer factor/T cell factor family, and modulation of gene expression. Axin is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3β. APC, which is phosphorylated by GSK-3β, induces degradation of β-catenin, thus inhibiting Wnt signal transduction. Conductin is 45% identical to Axin and appears to play a similar role to Axin in the Wnt signaling pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AXIN1 polyclonal antibody detects endogenous levels of AXIN1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human AXIN1. Conjugate :
Unconjugated Modification :
Unmodification
AXIN1 polyclonal antibody Background :
β-catenin is a component of both the cadherin cell adhesion system and the Wnt signaling pathway. Wnt signaling increases the amount of β-catenin by preventing its ubiquitination and degradation, allowing its direct interaction with transcription factors of the lymphoid enhancer factor/T cell factor family, and modulation of gene expression. Axin is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3β. APC, which is phosphorylated by GSK-3β, induces degradation of β-catenin, thus inhibiting Wnt signal transduction. Conductin is 45% identical to Axin and appears to play a similar role to Axin in the Wnt signaling pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AXIN1 polyclonal antibody detects endogenous levels of AXIN1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human AXIN1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of AXIN1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.