Product Name :
LIF polyclonal antibody Background :
Embryonic stem (ES) cells are the focus of much research and represent great therapeutic potential as they can be propagated indefinitely in an undifferentiated state while possessing the ability to differentiate into all embryonic germ layers (endoderm, ectoderm and mesoderm) both in vivo and in vitro. LIF (leukemia inhibitory factor), also known as MLPLI (melanoma-derived LPL inhibitor), HILDA, DIA or CDF, is a 202 amino acid secreted protein and lymphoid factor that participates in the maintenance of ES cell pluripotency by suppressing spontaneous ES cell differentiation. Secreted LIF precursor is further processed into a biologically active glycoprotein. Expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells, LIF is suggested to promote survival and growth of axons in vitro and is involved in immune tolerance at the maternal-fetal interface. LIF may also participate in fat and bone metabolism and regulate epithelial conversion during kidney development. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
LIF polyclonal antibody detects endogenous levels of LIF protein. Immunogen :
A synthetic peptide corresponding to residues in Human LIF. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of LIF polyclonal antibody at 1:500 dilution Lane1:Jurkat whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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