Product Name :
AANAT polyclonal antibody Background :
AANAT (Aralkylamine N-acetyltransferase), also called Serotonin N-acetyltransferase (SNAT) or Serotonin acetylase, is a member of the acetyltransferase superfamily. It is almost exclusively expressed in the pineal gland and the retina. AANAT activity is high at night and low during the day due to the 10 to 100-fold increase in the amount of active AANAT at night. Retinal exposure to light causes cAMP levels to decrease in photoreceptor cells and, as a result, AANAT is targeted for degradation by proteasomal proteolysis. AANAT plays an important role as the rate limiting enzyme in melatonin synthesis. It is responsible for catalyzing the N-acetylation of serotonin to N-acetylserotonin, which is then converted to melatonin by hydroxyindole-O-methyltransferase. Melatonin is an important hormone that is involved in many physiological processes including immune function, seasonal reproduction, retinal physiology and circadian entrainment. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AANAT polyclonal antibody detects endogenous levels of AANAT protein. Immunogen :
A synthetic peptide corresponding to residues in Human AANAT Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of AANAT polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:PC12 whole cell lysate
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  Immunohistochemistry (IHC) analyzes of AANAT pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic strong positive staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of AANAT pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic strong positive staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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