PRSS8 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
PRSS8 polyclonal antibody Background :
Prostasin, a serine protease first identified in prostate tissue, activates epithelial sodium channels in a variety of tissues. Though typically a membrane-anchored protein, free prostasin is also found in physiologic fluids and tissue culture media, indicating a mechanism for secretion from the cells as well. Aprotinin and other protease inhibitors suppress the channel-activating capacity of prostasin, while aldosterone increases prostasin expression and stimulates sodium uptake. In addition, DNA methylation negatively correlates with prostasin expression in cancer cells, while enforced reexpression decreases invasiveness as well as metastatic potential, implying that prostasin activity reflects epithelial cell physiology. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PRSS8 polyclonal antibody detects endogenous levels of PRSS8 protein. Immunogen :
A synthetic peptide corresponding to residues in Human PRSS8 Conjugate :
Unconjugated Modification :
Unmodification
PRSS8 polyclonal antibody Background :
Prostasin, a serine protease first identified in prostate tissue, activates epithelial sodium channels in a variety of tissues. Though typically a membrane-anchored protein, free prostasin is also found in physiologic fluids and tissue culture media, indicating a mechanism for secretion from the cells as well. Aprotinin and other protease inhibitors suppress the channel-activating capacity of prostasin, while aldosterone increases prostasin expression and stimulates sodium uptake. In addition, DNA methylation negatively correlates with prostasin expression in cancer cells, while enforced reexpression decreases invasiveness as well as metastatic potential, implying that prostasin activity reflects epithelial cell physiology. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PRSS8 polyclonal antibody detects endogenous levels of PRSS8 protein. Immunogen :
A synthetic peptide corresponding to residues in Human PRSS8 Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PRSS8 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.