RASIP1 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RASIP1 polyclonal antibody Background :
RAIN, also known as RASIP1 (Ras-interacting protein 1), is a 963 amino acid protein that contains one dilute domain and one Ras-associating domain. The RAIN protein is highly expressed in heart and detected at lower levels in placenta and pancreas. RAIN interacts with H-Ras, Rap 1A, Rap 2, R-Ras, Raf-1 and TC 21, as well as with Ras family members that have been activated by GTP binding. RAIN interacts with Ras in a GTP-dependent manner in vitro and in vivo, requiring an intact Ras core effector-binding domain for this interaction, and thus fits the definition of a Ras effector. Unlike other Ras effectors, RAIN localizes to the perinuclear, juxta-Golgi vesicles in intact cells and is recruited to the Golgi by activated Ras. Containing twelve exons, the RAIN gene is conserved in dog, cow, mouse, rat, chicken and zebrafish, and maps to human chromosome 19q13.33. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RASIP1 polyclonal antibody detects endogenous levels of RASIP1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RASIP1 Conjugate :
Unconjugated Modification :
Unmodification
RASIP1 polyclonal antibody Background :
RAIN, also known as RASIP1 (Ras-interacting protein 1), is a 963 amino acid protein that contains one dilute domain and one Ras-associating domain. The RAIN protein is highly expressed in heart and detected at lower levels in placenta and pancreas. RAIN interacts with H-Ras, Rap 1A, Rap 2, R-Ras, Raf-1 and TC 21, as well as with Ras family members that have been activated by GTP binding. RAIN interacts with Ras in a GTP-dependent manner in vitro and in vivo, requiring an intact Ras core effector-binding domain for this interaction, and thus fits the definition of a Ras effector. Unlike other Ras effectors, RAIN localizes to the perinuclear, juxta-Golgi vesicles in intact cells and is recruited to the Golgi by activated Ras. Containing twelve exons, the RAIN gene is conserved in dog, cow, mouse, rat, chicken and zebrafish, and maps to human chromosome 19q13.33. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RASIP1 polyclonal antibody detects endogenous levels of RASIP1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RASIP1 Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RASIP1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:H9C2 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.