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TPRA1 polyclonal antibody BS60833
  • Western blot (WB) analysis of TPRA1 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:MCF-7 whole cell lysate
Product NameTPRA1 polyclonal antibody
Catalog No.BS60833
Swiss-ProtQ86W33
Host Rabbit
ReactivityHuman
ApplicationsWB
Application_allWB: 1:500~1:1000
BiowMW~ 41 kDa
Alternative NameTransmembrane protein adipocyte-associated 1; Integral membrane protein GPR175; Transmembrane protein 227; TPRA1; GPR175; TMEM227; PP6566
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
TPRA1 polyclonal antibody
Background :
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR175 (G protein-coupled receptor 175), also known as TPRA40 or PP6566, is a 373 amino acid multi-pass membrane protein that is ubiquitously expressed and functions as a G-protein coupled receptor. The gene encoding GPR175 maps to human chromosome 3 and is expressed as multiple alternatively spliced isoforms.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
TPRA1 polyclonal antibody detects endogenous levels of TPRA1 protein.
Immunogen :
A synthetic peptide corresponding to residues in Human TPRA1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of TPRA1 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:MCF-7 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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