RIPK3 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
RIPK3 polyclonal antibody Background :
The death domain is a cytoplasmic domain of approximately 80 amino acids that is necessary for the transduction of apoptotic signals and is present in the apoptosis-mediating receptors TNF-R1 and FAS. Other death domain-containing, but otherwise structurally unrelated proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNF-R1 or FAS. One of these proteins, the receptor-interacting protein 3 (RIP3), contains an N-terminal kinase domain and shares extensive homology with RIP and RIP2. However, RIP3 contains a unique C-terminal death domain, which promotes apoptosis. RIP3 can be expressed as two splice variants, RIP3∫ and RIP3©, which contain a truncated N-terminal kinase domain and a distinct and shorter C-terminus. Subsequently, expression of these splice variants downregulates RIP3-mediated apoptosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RIPK3 polyclonal antibody detects endogenous levels of RIPK3 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RIPK3. Conjugate :
Unconjugated Modification :
Unmodification
RIPK3 polyclonal antibody Background :
The death domain is a cytoplasmic domain of approximately 80 amino acids that is necessary for the transduction of apoptotic signals and is present in the apoptosis-mediating receptors TNF-R1 and FAS. Other death domain-containing, but otherwise structurally unrelated proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNF-R1 or FAS. One of these proteins, the receptor-interacting protein 3 (RIP3), contains an N-terminal kinase domain and shares extensive homology with RIP and RIP2. However, RIP3 contains a unique C-terminal death domain, which promotes apoptosis. RIP3 can be expressed as two splice variants, RIP3∫ and RIP3©, which contain a truncated N-terminal kinase domain and a distinct and shorter C-terminus. Subsequently, expression of these splice variants downregulates RIP3-mediated apoptosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RIPK3 polyclonal antibody detects endogenous levels of RIPK3 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RIPK3. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RIPK3 polyclonal antibody at 1:500 dilution Lane1:Panc1 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.