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ALPP polyclonal antibody BS60863
  • Western blot (WB) analysis of ALPP polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate
Product NameALPP polyclonal antibody
Catalog No.BS60863
Swiss-ProtP05187/P10696
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB
Application_allWB: 1:500~1:1000
BiowMW~ 76 kDa
Alternative NameAlkaline phosphatase, placental type (EC:3.1.3.1); Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; ALPP; PLAP; ALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like; PLAP-like; ALPPL2; ALPPL
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
ALPP polyclonal antibody
Background :
Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+-metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. Placental alkaline phosphatase (also known as PLAP, ALPP, PALP, placental ALP-1 or Regan isozyme) is a 530 amino acid, tissue-specific AP that is expressed in the placenta, the serum of pregnant women and ectopically expressed in various cancers, including those of the ovary and testis. PLAP may assist in guiding migratory cells and transporting specific molecules, such as fatty acids and immunoglobulins, across the plasma membrane. The three tissue-specific APs identified in human, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous and their genes are clustered on chromosome 2q.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
ALPP polyclonal antibody detects endogenous levels of ALPP protein, and also detects ALPP2 protein.
Immunogen :
A synthetic peptide corresponding to residues in Human ALPP.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of ALPP polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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