IL2RA polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
IL2RA polyclonal antibody Background :
The IL-2 receptor is amulticomponentcomplexconsisting of three subunits,α, β and γ, each ofwhich isrequired for high-affinity binding of IL-2. Theαchain functions primarilyin binding IL-2, whereasthe β and γ chainscontribute to IL-2 binding and are essential to IL-2-induced activation ofsignaling pathways leading to T cell growth. Both IL-4R and IL-7R were initially described as single chain, high-affinityligand-binding cytokine receptors. However, it is now well established that the IL-2Rγ chain functions as a second subunit of the high affinityIL-4R and IL-7R receptors. Consequently, the originally described subunits of these latter receptors are nowreferred to asIL-4Rαand IL-7Rα, respectively,while the common subunit isreferred to as γc. Although the common γ chain enhancesligand binding in these three cytokine receptors, it has no capacityto bind these ligands on its own. There is evidence that the γcchain is also a subunit of IL-13R. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IL2RA polyclonal antibody detects endogenous levels of IL2RA protein. Immunogen :
A synthetic peptide corresponding to residues in Human IL2RA. Conjugate :
Unconjugated Modification :
Unmodification
IL2RA polyclonal antibody Background :
The IL-2 receptor is amulticomponentcomplexconsisting of three subunits,α, β and γ, each ofwhich isrequired for high-affinity binding of IL-2. Theαchain functions primarilyin binding IL-2, whereasthe β and γ chainscontribute to IL-2 binding and are essential to IL-2-induced activation ofsignaling pathways leading to T cell growth. Both IL-4R and IL-7R were initially described as single chain, high-affinityligand-binding cytokine receptors. However, it is now well established that the IL-2Rγ chain functions as a second subunit of the high affinityIL-4R and IL-7R receptors. Consequently, the originally described subunits of these latter receptors are nowreferred to asIL-4Rαand IL-7Rα, respectively,while the common subunit isreferred to as γc. Although the common γ chain enhancesligand binding in these three cytokine receptors, it has no capacityto bind these ligands on its own. There is evidence that the γcchain is also a subunit of IL-13R. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IL2RA polyclonal antibody detects endogenous levels of IL2RA protein. Immunogen :
A synthetic peptide corresponding to residues in Human IL2RA. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of IL2RA polyclonal antibody at 1:500 dilution Lane1:Thp-1 whole cell lysate Lane2:H9C2 whole cell lysate Lane3:Raw264.7 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.