Product Name :
RPS27L polyclonal antibody Background :
Ribosomal protein S27-like (RPS27L) is a homologue of 40S ribosomal subunit protein S27 (RPS27) with an unknown function. Using microarray analysis, RPS27L was found to be consistantly up-regulated in 5-fluorouracil (5-FU) and adriamycin (ADR)-treated colorectal HCT116 cells in a p53-dependent manner. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RPS27L pAb detects endogenous levels of RPS27L protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1035-1095 of Human MAST3. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of RPS27L pAb at 1:500 dilution Lane1:The Kidney tissue lysate of Mouse(40ug) Lane2:The Kidney tissue lysate of Rat(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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