PKM2 polyclonal antibody 
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Product Name :
PKM2 polyclonal antibody Background :
The PKM2 (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PKM2 polyclonal antibody detects endogenous levels of PKM2 protein. Immunogen :
Recombinant full length Human PKM2. Conjugate :
Unconjugated Modification :
Unmodification
PKM2 polyclonal antibody Background :
The PKM2 (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PKM2 polyclonal antibody detects endogenous levels of PKM2 protein. Immunogen :
Recombinant full length Human PKM2. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PKM2 polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The stomach tissue lysate of Mouse(40ug) Lane4:MCF-7 whole cell lysate(20ug) -
Immunohistochemistry (IHC) analysis of PKM2 pAb in paraffin-embedded Lung cancer tissue. -
Immunohistochemistry (IHC) analysis of PKM2 pAb in paraffin-embedded Lung cancer tissue.
Depletion of PKM2 leads to impaired glycolysis and cell death in 2-demethoxy-2, 3-ethylenediamino hypocrellin B-photoinduced A549 cells
PMCID: Pubmed No.:24142698
Oroxylin A regulates glucose metabolism in response to hypoxic stress with the involvement of Hypoxia‐inducible factor‐1 in human hepatoma HepG2 cells
PMCID: Pubmed No.:26259145
Metformin prevents DMH‐induced colorectal cancer in diabetic rats by reversing the warburg effect
PMCID: Pubmed No.:26376762
Metformin prevents DMH‐induced colorectal cancer in diabetic rats by reversing the warburg effect
PMCID: Pubmed No.:26376762
Depletion of PKM2 leads to impaired glycolysis and cell death in 2-demethoxy-2, 3-ethylenediamino hypocrellin B-photoinduced A549 cells
PMCID: Pubmed No.:24142698
Oxidative stress induced autophagy in cancer associated fibroblast enhances proliferation and metabolism of colorectal cancer cells
PMCID: Pubmed No.:27841696
Oroxylin A regulates glucose metabolism in response to hypoxic stress with the involvement of Hypoxia‐inducible factor‐1 in human hepatoma HepG2 cells
PMCID: Pubmed No.:26259145
Metformin prevents DMH‐induced colorectal cancer in diabetic rats by reversing the warburg effect
PMCID: Pubmed No.:26376762
The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality
PMCID: Pubmed No.:30786262
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.