Product Name :
AIF1 polyclonal antibody Background :
Iba1, also designated AIF-1 for allograft inflammatory factor-1, is a cytoplasmic, cytokine-induced, calcium-binding protein first discovered in rat cardiac allografts with chronic rejection. Iba1 is expressed in various human immune-related tissues and may play a role in macrophage activation and function. It is a marker of human microglia and is expressed by macrophages in injured skeletal muscle. Iba1 regulates reduced proliferation and differentiation of satellite cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AIF1 polyclonal antibody detects endogenous levels of AIF1 protein. Immunogen :
Recombinant full length Human AIF1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of AIF1 polyclonal antibody at 1:500 dilution Lane1:Jurkat whole cell lysate(40ug) Lane2:The Thymus tissue lysate of Mouse(40ug) Lane3:The Spleen tissue lysate of Rat(40ug) Lane4:RAW264.7 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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