p21 (FL) polyclonal antibody 
Datasheet
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MSDS
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Product Name :
p21 (FL) polyclonal antibody Background :
It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p21 (FL) polyclonal antibody detects endogenous levels of p21 protein. Immunogen :
Recombinant full length Human P21. Conjugate :
Unconjugated Modification :
Unmodification
p21 (FL) polyclonal antibody Background :
It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p21 (FL) polyclonal antibody detects endogenous levels of p21 protein. Immunogen :
Recombinant full length Human P21. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of p21 (FL) polyclonal antibody
Oxidized Low-Density Lipoprotein Induces Hematopoietic Stem Cell Senescence
PMCID: Pubmed No.:23620010
Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells.
PMCID: Pubmed No.:26580119
Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells.
PMCID: Pubmed No.:26580119
All‑trans‑retinoic acid inhibits chondrogenesis of rat embryo hindlimb bud mesenchymal cells by downregulating p53 expression
PMCID: Pubmed No.:25738595
Tumor necrosis factor-α promotes hepatocellular carcinogenesis through the activation of hepatic progenitor cells
PMCID: Pubmed No.:29981431
UBN2 promotes tumor progression via the Ras/MAPK pathway and predicts poor prognosis in colorectal cancer
PMCID: Pubmed No.:31110467
Role of DNA damage in the progress of chronic tubule‑interstitial injury
PMCID: Pubmed No.:32626982
Krüppel-like Factor 7 inhibits proliferation and migration of pulmonary smooth muscle cells via p21 activation
PMCID: Pubmed No.:36566916
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.