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Actived-Caspase-3 p17 polyclonal antibody BS7004
  • Western blot (WB) analysis of Actived-Caspase-3 p17 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)
  • Immunohistochemistry (IHC) analyzes of Actived-Caspase-3 p17 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameActived-Caspase-3 p17 polyclonal antibody
Catalog No.BS7004
Swiss-ProtP42574
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
BiowMW~ 17, 35 kDa
Alternative NameCaspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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100ul $298
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Product Name :
Actived-Caspase-3 p17 polyclonal antibody
Background :
Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10).The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF).
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Actived-Caspase-3 p17 polyclonal antibody detects endogenous levels of Actived-Caspase-3 p17 protein.
Immunogen :
Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 3.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Actived-Caspase-3 p17 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)
  • Immunohistochemistry (IHC) analyzes of Actived-Caspase-3 p17 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Suppression of epithelial–mesenchymal transition and apoptotic pathways by miR-294/302 family synergistically blocks let-7-induced silencing of self-renewal in embryonic stem cells PMCID:    Pubmed No.:25501598 Loss of KLF14 triggers centrosome amplification and tumorigenesis PMCID:    Pubmed No.:26439168 Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells PMCID:    Pubmed No.:27463126 CHAC2, downregulated in gastric and colorectal cancers, acted as a tumor suppressor inducing apoptosis and autophagy through unfolded protein response PMCID:    Pubmed No.:28837156 Platinum-containing compound platinum pyrithione suppresses ovarian tumor proliferation through proteasome inhibition PMCID:    Pubmed No.:28619062 Bamboo leaf extract ameliorates diabetic nephropathy through activating the AKT signaling pathway in rats PMCID:    Pubmed No.:28359892 Amifostine Pretreatment Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Apoptosis and Oxidative Stress PMCID:    Pubmed No.:28392886 Adiponectin reduces ER stress-induced apoptosis through PPAR α transcriptional regulation of ATF2 in mouse adipose PMCID:    Pubmed No.:27882945 Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease PMCID:    Pubmed No.:26203862 RN181 suppressed tumor growth of hepatocellular carcinoma by inhibition of the ERK/MAPK pathway PMCID:    Pubmed No.:21391225 ZL11n is a novel nitric oxide-releasing derivative of farnesylthiosalicylic acid that induces apoptosis in human hepatoma HepG2 cells via MAPK/mitochondrial pathways PMCID:    Pubmed No.:21621522 Free-Radical Scavenger Edaravone Treatment Confers Neuroprotection Against Traumatic Brain Injury in Rats PMCID:    Pubmed No.:21732763 Mesenchymal stem cells derived from Wharton jelly of the human umbilical cord ameliorate damage to human endometrial stromal cells PMCID:    Pubmed No.:21802667 HnRNPL as a key factor in spermatogenesis: Lesson from functional proteomic studies of azoospermia patients with sertoli cell only syndrome PMCID:    Pubmed No.:22245417 MicroRNA-409-3p regulates cell proliferation and apoptosis by targeting PHF10 in gastric cancer PMCID:    Pubmed No.:22388101 Reactive oxygen species-mediated mitochondrial pathway is involved in Baohuoside I-induced apoptosis in human non-small cell lung cancer PMCID:    Pubmed No.:22687635 Effects of PEG-liposomal oxaliplatin on apoptosis, and expression of Cyclin A and Cyclin D1 in colorectal cancer cells. PMCID:    Pubmed No.:22710431 Activation of nuclear factor erythroid 2-related factor 2 and PPARγ plays a role in the genistein-mediated attenuation of oxidative stress-induced endothelial cell injury PMCID:    Pubmed No.:22716961 NG, a novel PABA/NO-based oleanolic acid derivative, induces human hepatoma cell apoptosis via a ROS/MAPK- dependent mitochondrial pathway PMCID:    Pubmed No.:22824464 Isoorientin induces apoptosis through mitochondrial dysfunction and inhibition of PI3K/Akt signaling pathway in HepG2 cancer cells PMCID:    Pubmed No.:23026832 MicroRNA-145 Protects Cardiomyocytes against Hydrogen Peroxide (H2O2)-Induced Apoptosis through Targeting the Mitochondria Apoptotic Pathway PMCID:    Pubmed No.:23028672 Phycocyanin may suppress d-galactose-induced human lens epithelial cell apoptosis through mitochondrial and unfolded protein response pathways PMCID:    Pubmed No.:23036742 Inhibition of autophagy enhances anticancer effects of bevacizumab in hepatocarcinoma PMCID:    Pubmed No.:23052483 Herbacetin induces apoptosis in HepG2 Cells: Involvements of ROS and PI3K/Akt pathway PMCID:    Pubmed No.:23063593 T63, a New 4-Arylidene Curcumin Analogue induces cell cycle arrest and apoptosis through activation of Reactive Oxygen Species-FOXO3a pathway in lung cancer cells PMCID:    Pubmed No.:23085518 The Combined Effects of Hematoporphyrin Monomethyl Ether-SDT and Doxorubicin on the Proliferation of QBC939 Cell Lines PMCID:    Pubmed No.:23200181 MAPK signaling pathways regulate mitochondrial-mediated apoptosis induced by isoorientin in human hepatoblastoma cancer cells PMCID:    Pubmed No.:23220614 Osteopontin Splice Variants Differentially Exert Clinicopathological Features and Biological Functions in Gastric Cancer PMCID:    Pubmed No.:23289017 Functional graphene oxide as a plasmid-based Stat3 siRNA carrier inhibits mouse malignant melanoma growth in vivo PMCID:    Pubmed No.:23425941 [Gly14]-Humanin Offers Neuroprotection through Glycogen Synthase Kinase-3β Inhibition in a Mouse Model of Intracerebral Hemorrhage PMCID:    Pubmed No.:23538063 Deficiency of senescence marker protein 30 exacerbates angiotensin II-induced cardiac remodeling PMCID:    Pubmed No.:23933320 Salvianolic acid A preconditioning confers protection against concanavalin A-induced liver injury through SIRT1-mediated repression of p66shc in mice PMCID:    Pubmed No.:23993977 ONTD induces apoptosis of human hepatoma Bel-7402 cells via a MAPK-dependent mitochondrial pathway and the depletion of intracellular glutathione PMCID:    Pubmed No.:24036456 Anti-Necroptosis Chemical Necrostatin-1 Can Also Suppress Apoptotic and Autophagic Pathway to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model. PMCID:    Pubmed No.:24122153 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:None The Combination of Proteasome Inhibitors Bortezomib and Gambogic Acid Triggers Synergistic Cytotoxicity in vitro but not in vivo PMCID:    Pubmed No.:24291039 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:24335166 Additive effect of zinc oxide nanoparticles and isoorientin on apoptosis in human hepatoma cell line PMCID:    Pubmed No.:24374571 Calcium channel blocker verapamil accelerates gambogic acid-induced cytotoxicity via enhancing proteasome inhibition and ROS generation PMCID:    Pubmed No.:24373880 Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells PMCID:    Pubmed No.:24466342 Endoplasmic Reticulum Stress-Unfolding Protein Response-Apoptosis Cascade Causes Chondrodysplasia in a col2a1 p. Gly1170Ser Mutated Mouse Model PMCID:    Pubmed No.:24475193 Gastrodin Inhibits Glutamate-Induced Apoptosis of PC12 Cells via Inhibition of CaMKII/ASK-1/p38 MAPK/p53 Signaling Cascade PMCID:    Pubmed No.:24619207 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Selective inhibition of protein kinase C β2 attenuates the adaptor P66Shc-mediated intestinal ischemia–reperfusion injury PMCID:    Pubmed No.:24722289 Airborne fine particulate matter induces multiple cell death pathways in human lung epithelial cells PMCID:    Pubmed No.:24722831 Hydrogen sulfide attenuates hypoxia-induced neurotoxicity through inhibiting microglial activation PMCID:    Pubmed No.:24788079 Paraspeckle Protein 1 (PSPC1) Is Involved in the Cisplatin Induced DNA Damage Response—Role in G1/S Checkpoint PMCID:    Pubmed No.:24819514 Isoorientin induces apoptosis and autophagy simultaneously by ROS-related P53, PI3K/Akt, JNK and P38 signaling pathways in HepG2 cancer cells PMCID:    Pubmed No.:24841907 Blockade of PKCβ protects against remote organ injury induced by intestinal ischemia and reperfusion via a p66shc-mediated mitochondrial apoptotic pathway PMCID:    Pubmed No.:24930012 Mark4 promotes adipogenesis and triggers apoptosis in 3T3-L1 adipocytes by activating JNK1 and inhibiting p38MAPK pathways. PMCID:    Pubmed No.:24989893 Levofloxacin increases the effect of serum deprivation on anoikis of rat nucleus pulposus cells via Bax/Bcl-2/caspase-3 pathway. PMCID:    Pubmed No.:25224805 Suppression of epithelial-mesenchymal transition and apoptotic pathways by miR-294/302 family synergistically blocks let-7-induced silencing of self-renewal in embryonic stem cells. PMCID:    Pubmed No.:25501598 Methylmercury causes neuronal cell death through the suppression of the TrkA pathway: In vitro and in vivo effects of TrkA pathway activators. PMCID:    Pubmed No.:25545984 Protective efficacy of carnosic acid against hydrogen peroxide (H2O2)-induced oxidative injury in HepG2 cells through SIRT1 pathway PMCID:    Pubmed No.:26059423 Activation of cellular apoptosis in the cecal epithelium is associated with increased oxidative reactions in lactating goats following high concentrate diet feeding PMCID:    Pubmed No.:25590230 Neuroprotection by peripheral nerve electrical stimulation and remote postconditioning against acute experimental ischaemic stroke PMCID:    Pubmed No.:25819636 NOAD, a novel nitric oxide donor, induces G2/M phase arrest and apoptosis in human hepatocellular carcinoma Bel-7402 cells PMCID:    Pubmed No.:26001517 Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mice pre-adipocyte PMCID:    Pubmed No.:26113535 The Orphan Nuclear Receptor NR4A1 Protects Pancreatic β-cells from ER-Stress-Mediated Apoptosis. PMCID:    Pubmed No.:26157144 Salinomycin radiosensitizes human nasopharyngeal carcinoma cell line CNE-2 to radiation PMCID:    Pubmed No.:26209294 Inhibitory effect of epirubicin-loaded lipid microbubbles with conjugated anti-ABCG2 antibody combined with therapeutic ultrasound on multiple myeloma cancer stem cells. PMCID:    Pubmed No.:26204324 Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease PMCID:    Pubmed No.:26203862 Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes PMCID:    Pubmed No.:26257270 Tert-butylhydroquinone Ameliorates Early Brain Injury After Experimental Subarachnoid Hemorrhage in Mice by Enhancing Nrf2-Independent Autophagy PMCID:    Pubmed No.:26260377 Loss of KLF14 triggers centrosome amplification and tumorigenesis. PMCID:    Pubmed No.:26439168 The molecular mechanisms of XBP-1 gene silencing on IRE1α-TRAF2-ASK1-JNK pathways in oral squamous cell carcinoma under endoplasmic reticulum stress PMCID:    Pubmed No.:26796273 The Pivotal Role of Ca2+ Homeostasis in PBDE-47-Induced Neuronal Apoptosis PMCID:    Pubmed No.:26676572 STAT3 degradation mediated by calcineurin involved in the neurotoxicity of isoflurane PMCID:    Pubmed No.:26694943 Persistent mitoKATP Activation Is Involved in the Isoflurane-induced Cytotoxicity PMCID:    Pubmed No.:26803495 Blocking B7-1/CD28 Pathway Diminished Long-Range Brain Damage by Regulating the Immune and Inflammatory Responses in a Mouse Model of Intracerebral Hemorrhage. PMCID:    Pubmed No.:26980009 Autophagy Regulates Intracerebral Hemorrhage Induced Neural Damage via Apoptosis and NF-κB Pathway PMCID:    Pubmed No.:26964766 A novel nickel complex works as a proteasomal deubiquitinase inhibitor for cancer therapy PMCID:    Pubmed No.:27086925 Levofloxacin increases the effect of serum deprivation on anoikis of rat nucleus pulposus cells via Bax/Bcl-2/caspase-3 pathway. PMCID:    Pubmed No.:25224805 Suppression of epithelial-mesenchymal transition and apoptotic pathways by miR-294/302 family synergistically blocks let-7-induced silencing of self-renewal in embryonic stem cells. PMCID:    Pubmed No.:25501598 Methylmercury causes neuronal cell death through the suppression of the TrkA pathway: In vitro and in vivo effects of TrkA pathway activators. PMCID:    Pubmed No.:25545984 Anti-Necroptosis Chemical Necrostatin-1 Can Also Suppress Apoptotic and Autophagic Pathway to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model. PMCID:    Pubmed No.:24122153 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:None Loss of KLF14 triggers centrosome amplification and tumorigenesis. PMCID:    Pubmed No.:26439168 The molecular mechanisms of XBP-1 gene silencing on IRE1α-TRAF2-ASK1-JNK pathways in oral squamous cell carcinoma under endoplasmic reticulum stress PMCID:    Pubmed No.:26796273 The Pivotal Role of Ca2+ Homeostasis in PBDE-47-Induced Neuronal Apoptosis PMCID:    Pubmed No.:26676572 STAT3 degradation mediated by calcineurin involved in the neurotoxicity of isoflurane PMCID:    Pubmed No.:26694943 Persistent mitoKATP Activation Is Involved in the Isoflurane-induced Cytotoxicity PMCID:    Pubmed No.:26803495 Blocking B7-1/CD28 Pathway Diminished Long-Range Brain Damage by Regulating the Immune and Inflammatory Responses in a Mouse Model of Intracerebral Hemorrhage. PMCID:    Pubmed No.:26980009 Autophagy Regulates Intracerebral Hemorrhage Induced Neural Damage via Apoptosis and NF-κB Pathway PMCID:    Pubmed No.:26964766 A novel nickel complex works as a proteasomal deubiquitinase inhibitor for cancer therapy PMCID:    Pubmed No.:27086925 Hydrogen sulfide attenuates hypoxia-induced neurotoxicity through inhibiting microglial activation PMCID:    Pubmed No.:24788079 Paraspeckle Protein 1 (PSPC1) Is Involved in the Cisplatin Induced DNA Damage Response—Role in G1/S Checkpoint PMCID:    Pubmed No.:24819514 The Combination of Proteasome Inhibitors Bortezomib and Gambogic Acid Triggers Synergistic Cytotoxicity in vitro but not in vivo PMCID:    Pubmed No.:24291039 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:24335166 Additive effect of zinc oxide nanoparticles and isoorientin on apoptosis in human hepatoma cell line PMCID:    Pubmed No.:24374571 Calcium channel blocker verapamil accelerates gambogic acid-induced cytotoxicity via enhancing proteasome inhibition and ROS generation PMCID:    Pubmed No.:24373880 Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells PMCID:    Pubmed No.:24466342 Endoplasmic Reticulum Stress-Unfolding Protein Response-Apoptosis Cascade Causes Chondrodysplasia in a col2a1 p. Gly1170Ser Mutated Mouse Model PMCID:    Pubmed No.:24475193 Gastrodin Inhibits Glutamate-Induced Apoptosis of PC12 Cells via Inhibition of CaMKII/ASK-1/p38 MAPK/p53 Signaling Cascade PMCID:    Pubmed No.:24619207 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Selective inhibition of protein kinase C β2 attenuates the adaptor P66Shc-mediated intestinal ischemia–reperfusion injury PMCID:    Pubmed No.:24722289 Airborne fine particulate matter induces multiple cell death pathways in human lung epithelial cells PMCID:    Pubmed No.:24722831 Notch signaling plays a crucial role in cancer stem-like cells maintaining stemness and mediating chemotaxis in renal cell carcinoma PMCID:    Pubmed No.:28279221 Persistent mitoKATP Activation Is Involved in the Isoflurane-induced Cytotoxicity PMCID:    Pubmed No.:26803495 Methylation of DIRAS1 promotes colorectal cancer progression and may serve as a marker for poor prognosis PMCID:    Pubmed No.:28491151 Di-(2-ethylhexyl) phthalate induces apoptosis of GC-2spd cells via TR4/Bcl-2 pathway PMCID:    Pubmed No.:27084994 Enhancing the anti-multiple myeloma efficiency in a cancer stem cell xenograft model by conjugating the ABCG2 antibody with microbubbles for a targeted delivery of ultrasound mediated epirubicin PMCID:    Pubmed No.:28232025 The Pivotal Role of Ca2+ Homeostasis in PBDE-47-Induced Neuronal Apoptosis PMCID:    Pubmed No.:26676572 Diazoxide protects L6 skeletal myoblasts from H2O2-induced apoptosis via the phosphatidylinositol-3 kinase/Akt pathway PMCID:    Pubmed No.:26525360 Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes PMCID:    Pubmed No.:26113535 Zeaxanthin improves diabetes-induced cognitive deficit in rats through activiting PI3K/AKT signaling pathway PMCID:    Pubmed No.:28599877 RETRACTED ARTICLE: Advanced oxidation protein products induce chondrocyte death through a redox-dependent, poly (ADP-ribose) polymerase-1-mediated pathway PMCID:    Pubmed No.:27858200 NGF Attenuates High Glucose-Induced ER Stress, Preventing Schwann Cell Apoptosis by Activating the PI3K/Akt/GSK3β and ERK1/2 Pathways PMCID:    Pubmed No.:28762104 Autophagy regulates intracerebral hemorrhage induced neural damage via apoptosis and NF-κB pathway PMCID:    Pubmed No.:26964766 The mTOR promotes oxidative stress-induced apoptosis of mesangial cells in diabetic nephropathy PMCID:    Pubmed No.:29277549 A novel small molecule compound diaporine inhibits breast cancer cell proliferation via promoting ROS generation PMCID:    Pubmed No.:27543689 The molecular mechanisms of XBP-1 gene silencing on IRE1α-TRAF2-ASK1-JNK pathways in oral squamous cell carcinoma under endoplasmic reticulum stress PMCID:    Pubmed No.:26796273 Chronic intermittent hypoxia induces cardiac hypertrophy by impairing autophagy through the adenosine 5′-monophosphate-activated protein kinase pathway PMCID:    Pubmed No.:27412517 NOAD, a novel nitric oxide donor, induces G2/M phase arrest and apoptosis in human hepatocellular carcinoma Bel-7402 cells PMCID:    Pubmed No.:26001517 Downregulation of DEC1 contributes to the neurotoxicity induced by MPP+ by suppressing PI3K/Akt/GSK3β pathway PMCID:    Pubmed No.:28734031 Methylmercury causes neuronal cell death through the suppression of the TrkA pathway: In vitro and in vivo effects of TrkA pathway activators PMCID:    Pubmed No.:25545984 Up-regulation of Tight-Junction Proteins by p38 Mitogen-Activated Protein Kinase/p53 Inhibition Leads to a Reduction of Injury to the Intestinal Mucosal Barrier in Severe Acute Pancreatitis PMCID:    Pubmed No.:27171513 The Pyk2/MCU pathway in the rat middle cerebral artery occlusion model of ischemic stroke PMCID:    Pubmed No.:28916471 Inhibitory effect of epirubicin-loaded lipid microbubbles with conjugated anti-ABCG2 antibody combined with therapeutic ultrasound on multiple myeloma cancer stem cells PMCID:    Pubmed No.:26204324 Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes PMCID:    Pubmed No.:26257270 Annosquacin B induces mitochondrial apoptosis in multidrug resistant human breast cancer cell line MCF-7/ADR through selectively modulating MAPKs pathways PMCID:    Pubmed No.:27450387 Activation of cellular apoptosis in the caecal epithelium is associated with increased oxidative reactions in lactating goats after feeding a high-concentrate diet PMCID:    Pubmed No.:25590230 Equol Attenuates Atherosclerosis in Apolipoprotein E-Deficient Mice by Inhibiting Endoplasmic Reticulum Stress via Activation of Nrf2 in Endothelial Cells PMCID:    Pubmed No.:27907038 TRAF2 is a Valuable Prognostic Biomarker in Patients with Prostate Cancer PMCID:    Pubmed No.:28855498 Neuroprotection by peripheral nerve electrical stimulation and remote postconditioning against acute experimental ischaemic stroke PMCID:    Pubmed No.:25819636 Curcumin improves the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cells via the NF‑κB‑p53‑caspase‑3 pathway PMCID:    Pubmed No.:25780454 Triptolide exerts pro-apoptotic and cell cycle arrest activity on drug-resistant human lung cancer A549/Taxol cells via modulation of MAPK and PI3K/Akt signaling pathways PMCID:    Pubmed No.:27900040 Glycolysis inhibitor 2-deoxy-D-glucose suppresses carcinogen-induced rat hepatocarcinogenesis by restricting cancer cell metabolism PMCID:    Pubmed No.:25394852 17β-Estradiol Inhibites Tumor Necrosis Factor-α Induced Apoptosis of Human Nucleus Pulposus Cells via the PI3K/Akt Pathway PMCID:    Pubmed No.:27847386 Combined therapeutic effects of bortezomib and anacardic acid on multiple myeloma cells via activation of the endoplasmic reticulum stress response PMCID:    Pubmed No.:27430733 Emodin promotes the arrest of human lymphoma Raji cell proliferation through the UHRF1‑DNMT3A‑∆Np73 pathways PMCID:    Pubmed No.:28901428 Reducing Smad3/ATF4 was essential for Sirt1 inhibiting ER stress-induced apoptosis in mice brown adipose tissue PMCID:    Pubmed No.:28030827 A novel deubiquitinase inhibitor b-AP15 triggers apoptosis in both androgen receptor-dependent and -independent prostate cancers PMCID:    Pubmed No.:28968984 Novel mutations in the RB1 gene from Chinese families with a history of retinoblastoma PMCID:    Pubmed No.:25424699 Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis PMCID:    Pubmed No.:25520141 Epigenetic silencing of TMEM176A promotes esophageal squamous cell cancer development PMCID:    Pubmed No.:29050260 Hoxa5 increases mitochondrial apoptosis by inhibiting Akt/mTORC1/S6K1 pathway in mice white adipocytes PMCID:    Pubmed No.:29221131 Salinomycin radiosensitizes human nasopharyngeal carcinoma cell line CNE-2 to radiation PMCID:    Pubmed No.:26209294 Alteration of MDM2 by the Small Molecule YF438 Exerts Antitumor Effects in Triple-Negative Breast Cancer PMCID:    Pubmed No.:33985974 The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation PMCID:    Pubmed No.:33397932 Resveratrol improves human umbilical cord-derived mesenchymal stem cells repair for cisplatin-induced acute kidney injury PMCID:    Pubmed No.:30237401 Di(n-butyl) phthalate exposure impairs meiotic competence and development of mouse oocyte PMCID:    Pubmed No.:30605815 Arsenic and sulfur dioxide co-exposure induce renal injury via activation of the NF-κB and caspase signaling pathway PMCID:    Pubmed No.:30825854 Combination of the natural compound Periplocin and TRAIL induce esophageal squamous cell carcinoma apoptosis in vitro and in vivo: Implication in anticancer therapy PMCID:    Pubmed No.:31864387 Potent effects of dioscin against hepatocellular carcinoma through regulating TP53‐induced glycolysis and apoptosis regulator (TIGAR)‐mediated apoptosis, autophagy, and DNA damage PMCID:    Pubmed No.:30710454 MiR-16-5p targets SESN1 to regulate the p53 signaling pathway, affecting myoblast proliferation and apoptosis, and is involved in myoblast differentiation PMCID:    Pubmed No.:29511169 Recombinant angiopoietin-like protein 4 attenuates intestinal barrier structure and function injury after ischemia/reperfusion PMCID:    Pubmed No.:34539141 A new gold(I) complex-Au(PPh3)PT is a deubiquitinase inhibitor and inhibits tumor growth PMCID:    Pubmed No.:30527624 NOD2 promotes dopaminergic degeneration regulated by NADPH oxidase 2 in 6-hydroxydopamine model of Parkinson’s disease PMCID:    Pubmed No.:30157869 Pyridostigmine alleviates cardiac dysfunction via improving mitochondrial cristae shape in a mouse model of metabolic syndrome PMCID:    Pubmed No.:30633969 Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells PMCID:    Pubmed No.:33663206 Egg Yolk Immunoglobulin Supplementation Prevents Rat Liver from Aflatoxin B1-Induced Oxidative Damage and Genotoxicity PMCID:    Pubmed No.:30449111 The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling PMCID:    Pubmed No.:31884423 miR-103/107 promote ER stress-mediated apoptosis via targeting the Wnt3a/β-catenin/ATF6 pathway in preadipocytes PMCID:    Pubmed No.:29483204 Oroxylin A increases the sensitivity of temozolomide on glioma cells by hypoxia‐inducible factor 1α/hedgehog pathway under hypoxia PMCID:    Pubmed No.:30790292 γ-glutamylcysteine suppresses cadmium-induced apoptosis in PC12 cells via regulating oxidative stress PMCID:    Pubmed No.:34767868 Apoptosis Induction by Iron Radiation via Inhibition of Autophagy in Trp53+/-Mouse Testes: Is Chronic Restraint-Induced Stress a Modifying Factor? PMCID:    Pubmed No.:29989073 Retinol palmitate protects against myocardial ischemia/reperfusion injury via reducing oxidative stress and inhibiting apoptosis PMCID:    Pubmed No.:30972178 Human Umbilical Cord MSC-Derived Exosomes Suppress the Development of CCl4-Induced Liver Injury through Antioxidant Effect PMCID:    Pubmed No.:29686713 Control of preadipocyte proliferation, apoptosis and early adipogenesis by the forkhead transcription factor FoxO6 PMCID:    Pubmed No.:33290791 β-Elemene Reverses the Resistance of p53-Deficient Colorectal Cancer Cells to 5-Fluorouracil by Inducing Pro-death Autophagy and Cyclin D3-Dependent Cycle Arrest PMCID:    Pubmed No.:32457882 Phosphatidylinositol 3-Kinase/Akt signal pathway resists the apoptosis and inflammation in human extravillous trophoblasts induced by Porphyromonas gingivalis PMCID:    Pubmed No.:30448607 Neuroprotective effect of resveratrol against radiation after surgically induced brain injury by reducing oxidative stress, inflammation, and apoptosis through NRf2/HO‐1/NF‐κB signaling pathway PMCID:    Pubmed No.:32815255 Transcobalamin I: a novel prognostic biomarker of neoadjuvant chemotherapy in locally advanced hypopharyngeal squamous cell cancers PMCID:    Pubmed No.:30100732 Synergistic effect of glycated chitosan and photofrin photodynamic therapy on different breast tumor model PMCID:    Pubmed No.:32485403 Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis PMCID:    Pubmed No.:30994729 -Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells PMCID:    Pubmed No.:30363706 Neuroprotective effect of Ginsenoside Re against neurotoxin‑induced Parkinson's disease models via induction of Nrf2 PMCID:    Pubmed No.:35543148 CircPLXNA2 Affects the Proliferation and Apoptosis of Myoblast through circPLXNA2/gga-miR-12207-5P/MDM4 Axis PMCID:    Pubmed No.:36982536 Pioglitazone protects PC12 cells against oxidative stress injury: An in vitro study of its antiapoptotic effects via the PPARγ pathway PMCID:    Pubmed No.:37854503
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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