Product Name :
Lamin A/C (D230) polyclonal antibody Background :
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization. Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment. The cleavage of lamins results in nuclear disregulation and cell death. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Lamin A/C (D230) polyclonal antibody detects endogenous levels of Lamin A/C protein. Immunogen :
Synthetic peptide, corresponding to amino acids 231-280 of Human Lamin A. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Lamin A/C (D230) at 1:500 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analysis of Lamin A/C (D230) pAb in paraffin-embedded human lung carcinoma tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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