Cleaved-Notch 1 (V1754) polyclonal antibody 
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Product Name :
Cleaved-Notch 1 (V1754) polyclonal antibody Background :
Notch1 is a transmembrane protein functioning in development and the determination of cell fate. During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1753 and Val1754. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cleaved-Notch 1 (V1754) polyclonal antibody detects endogenous levels of the Notch1 intracellular domain (NICD) . The antibody does not recognize full-length Notch1. Immunogen :
Synthetic peptide, corresponding to amino acids 1700-1800 of Human Notch 1. Conjugate :
Unconjugated Modification :
Cleaved
Cleaved-Notch 1 (V1754) polyclonal antibody Background :
Notch1 is a transmembrane protein functioning in development and the determination of cell fate. During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1753 and Val1754. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cleaved-Notch 1 (V1754) polyclonal antibody detects endogenous levels of the Notch1 intracellular domain (NICD) . The antibody does not recognize full-length Notch1. Immunogen :
Synthetic peptide, corresponding to amino acids 1700-1800 of Human Notch 1. Conjugate :
Unconjugated Modification :
Cleaved
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Immunohistochemistry (IHC) analyzes of Cleaved-Notch 1 (V1754) pAb in paraffin-embedded human brain tissue.
Norisoboldine Suppresses VEGF-Induced Endothelial Cell Migration via the cAMP-PKA-NF-κB/Notch1 Pathway
PMCID: Pubmed No.:24349042
TGF-β1 pathway affects the protein expression of many signaling pathways, markers of liver cancer stem cells, cytokeratins, and TERT in liver cancer HepG2 cells
PMCID: Pubmed No.:26462837
TGF-β1 pathway affects the protein expression of many signaling pathways, markers of liver cancer stem cells, cytokeratins, and TERT in liver cancer HepG2 cells
PMCID: Pubmed No.:26462837
Norisoboldine Suppresses VEGF-Induced Endothelial Cell Migration via the cAMP-PKA-NF-κB/Notch1 Pathway
PMCID: Pubmed No.:24349042
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.