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BCL2 polyclonal antibody BS70205
  • Western blot analysis of extracts of various cell lines using BCL2 antibody.
  • Immunohistochemistry of paraffin-embedded human breast using BCL2 antibody at dilution of 1:100 (x40 lens).
  • Immunohistochemistry of paraffin-embedded human breast using BCL2 antibody at dilution of 1:100 (x40 lens).
Product NameBCL2 polyclonal antibody
Catalog No.BS70205
Swiss-ProtP10415
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF IP
Application_allWB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
BiowMW~ 26 kDa
Alternative NameBCL2;Bcl-2 ;ONCOGENE B-CELL LEUKEMIA 2; Apoptosis regulator Bcl-2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
BCL2 polyclonal antibody
Background :
Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
BCL2 polyclonal antibody detects endogenous levels of BCL2 protein.
Immunogen :
A synthetic peptide of human BCL2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of extracts of various cell lines using BCL2 antibody.
  • Immunohistochemistry of paraffin-embedded human breast using BCL2 antibody at dilution of 1:100 (x40 lens).
  • Immunohistochemistry of paraffin-embedded human breast using BCL2 antibody at dilution of 1:100 (x40 lens).
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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