Caspase 5 p20 (D121) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Caspase 5 p20 (D121) polyclonal antibody Background :
Caspase-5 (also designated ICErel-III, TY, ICH-3 and caspase-12 in mouse), can cleave its own precursor, an activity that requires the cysteine 245 residue. Frameshift mutations in caspase-5 have been identified in MMP tumors of the endometrium, colon, and stomach, indicating the caspase-5 may be a new target gene in the microsatellite mutator pathway for cancer. The human caspase 5 gene maps to chromosome 11q22.2-q22.3 and encodes a protein whose expression is barely detectable in most tissues except brain, with highest expression levels being found in lung, liver and skeletal muscle. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase-5 p20 (D121) polyclonal antibody detects endogenous levels of the large subunit of active caspase-5 resulting from cleavage at Asp121. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human Caspase-5. Conjugate :
Unconjugated Modification :
Unmodification
Caspase 5 p20 (D121) polyclonal antibody Background :
Caspase-5 (also designated ICErel-III, TY, ICH-3 and caspase-12 in mouse), can cleave its own precursor, an activity that requires the cysteine 245 residue. Frameshift mutations in caspase-5 have been identified in MMP tumors of the endometrium, colon, and stomach, indicating the caspase-5 may be a new target gene in the microsatellite mutator pathway for cancer. The human caspase 5 gene maps to chromosome 11q22.2-q22.3 and encodes a protein whose expression is barely detectable in most tissues except brain, with highest expression levels being found in lung, liver and skeletal muscle. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase-5 p20 (D121) polyclonal antibody detects endogenous levels of the large subunit of active caspase-5 resulting from cleavage at Asp121. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human Caspase-5. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Caspase-5 p20 (D121) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug)
Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice
PMCID: Pubmed No.:26434621
Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice
PMCID: Pubmed No.:26434621
Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice
PMCID: Pubmed No.:26434621
Ginsenoside Rg1 protects against neuronal degeneration induced by chronic dexamethasone treatment by inhibiting NLRP-1 inflammasomes in mice
PMCID: Pubmed No.:28849171
Axon guidance molecule semaphorin3A is a novel tumor suppressor in head and neck squamous cell carcinoma
PMCID: Pubmed No.:26755661
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.