CARP polyclonal antibody 
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Product Name :
CARP polyclonal antibody Background :
A proposed genetic marker of cardiac hypertrophy, CARP (cardiac ankyrin repeat protein, ANKRD1, Ankyrin repeat domain-containing protein 1,) is a nuclear protein with an established role in regulation of cardiac gene expression. A distinct increase in CARP expression occurs in rats with abdominal aorta constriction, spontaneous hypertension and Dahl salt-sensitivity. In cardiomyocytes, CARP inhibits transcription of both cardiac troponin C and atrial natriuretic factor. Specifically, expression of the CARP gene, which lies downstream of the cardiac homeobox gene Nkx2.5, inhibits Nkx2.5 transactivation of atrial natriuretic factor promoter. An increase in CARP expression is observed in the ventricular tissue of patients with endstage heart failure. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CARP polyclonal antibody detects endogenous levels of CARP protein. Immunogen :
Recombinant protein of human ANKRD1 Conjugate :
Unconjugated Modification :
Unmodification
CARP polyclonal antibody Background :
A proposed genetic marker of cardiac hypertrophy, CARP (cardiac ankyrin repeat protein, ANKRD1, Ankyrin repeat domain-containing protein 1,) is a nuclear protein with an established role in regulation of cardiac gene expression. A distinct increase in CARP expression occurs in rats with abdominal aorta constriction, spontaneous hypertension and Dahl salt-sensitivity. In cardiomyocytes, CARP inhibits transcription of both cardiac troponin C and atrial natriuretic factor. Specifically, expression of the CARP gene, which lies downstream of the cardiac homeobox gene Nkx2.5, inhibits Nkx2.5 transactivation of atrial natriuretic factor promoter. An increase in CARP expression is observed in the ventricular tissue of patients with endstage heart failure. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CARP polyclonal antibody detects endogenous levels of CARP protein. Immunogen :
Recombinant protein of human ANKRD1 Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of extracts of various cell lines, using ANKRD1 antibody at 1:1000 dilution.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.