ALKBH3 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ALKBH3 polyclonal antibody Background :
ALKBH3 (alkB, alkylation repair homolog 3), also known as ABH3, PCA1 (prostate cancer antigen 1), DEPC1 or DEPC-1, is a 286 amino acid member of the AlkB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. ALKBH3 is one of many homologs of the Escherichia coli protein, AlkB. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. It associates with iron and 2-oxoglutarate, coupling the oxidation of substrates to the conversion of 2-oxoglutarate into succinate and CO2. Via oxidative demethylation, ALKBH3 repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA. Its activity is stimulated by ascorbate. Two isoforms exist for ALKBH3 due to alternative splicing of the gene. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ALKBH3 polyclonal antibody detects endogenous levels of ALKBH3 protein. Immunogen :
Recombinant full length Human ALKBH3. Conjugate :
Unconjugated Modification :
Unmodification
ALKBH3 polyclonal antibody Background :
ALKBH3 (alkB, alkylation repair homolog 3), also known as ABH3, PCA1 (prostate cancer antigen 1), DEPC1 or DEPC-1, is a 286 amino acid member of the AlkB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. ALKBH3 is one of many homologs of the Escherichia coli protein, AlkB. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. It associates with iron and 2-oxoglutarate, coupling the oxidation of substrates to the conversion of 2-oxoglutarate into succinate and CO2. Via oxidative demethylation, ALKBH3 repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA. Its activity is stimulated by ascorbate. Two isoforms exist for ALKBH3 due to alternative splicing of the gene. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ALKBH3 polyclonal antibody detects endogenous levels of ALKBH3 protein. Immunogen :
Recombinant full length Human ALKBH3. Conjugate :
Unconjugated Modification :
Unmodification
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WesternBlot (WB) analysis of ALKBH3 polyclonal antibody
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.