ALIX polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ALIX polyclonal antibody Background :
ALG-2-interacting protein (Alix), also designated programmed cell death 6-interacting protein (PDCD6-interacting protein), is a cytoplasmic protein. Alix interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. Additionally, Alix interacts with the endosomal sorting complexes required for transport (ESCRT) proteins (Tsg101 and CHMP4) and can associate with HIV-1. The endophilins (SH3P4, SH3P8 and SH3P13), enzymes that change curvature of the membrane that are required for early and late steps of coated vesicle formation, also bind to Alix. Alix is involved in the concentration and sorting of cargo proteins of the multivesicular body for incorporation into vesicles. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
ALIX polyclonal antibody detects endogenous levels of ALIX protein. Immunogen :
Recombinant protein Conjugate :
Unconjugated Modification :
Unmodification
ALIX polyclonal antibody Background :
ALG-2-interacting protein (Alix), also designated programmed cell death 6-interacting protein (PDCD6-interacting protein), is a cytoplasmic protein. Alix interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. Additionally, Alix interacts with the endosomal sorting complexes required for transport (ESCRT) proteins (Tsg101 and CHMP4) and can associate with HIV-1. The endophilins (SH3P4, SH3P8 and SH3P13), enzymes that change curvature of the membrane that are required for early and late steps of coated vesicle formation, also bind to Alix. Alix is involved in the concentration and sorting of cargo proteins of the multivesicular body for incorporation into vesicles. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
ALIX polyclonal antibody detects endogenous levels of ALIX protein. Immunogen :
Recombinant protein Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of ALIX on different cell lysates using anti-ALIX antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: NIH-3T3 -
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ALIX antibody. Counter stained with hematoxylin.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.