Product Name :
Angiotensinogen polyclonal antibody Background :
Angiotensin is formed from a precursor, angiotensinogen, which is produced by the liver and found in the a-globulin fraction of plasma. The lowering of blood pressure is a stimulus to secretion of Renin by the kidney into the blood. Renin cleaves from angiotensinogen a terminal decapeptide, Angiotensin I (Ang I). This is further altered by the enzymatic removal of a dipeptide to form Angiotensin II (Ang II). Screening a panel of human-mouse somatic cell hybrids confirmed the assignment of the AGT locus to human chromosome 1. Angiotensin, an octapeptide hormone, is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. It has been shown that mechanical stress causes release of Angiotensin from cardiac myocytes and that Angiotensin acts as an initial mediator of the hypertrophic response. Angiotensin treatment also stimulates phosphorylation of Shc, FAK and MAP kinases and induces MKP-1, indicating stimulation of growth factor pathways. Angiotensin stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by co-immunoprecipitation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
Angiotensinogen polyclonal antibody detects endogenous levels of Angiotensinogen protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  ICC staining Angiotensinogen in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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  ICC staining Angiotensinogen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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