Product Name :
ASNS polyclonal antibody Background :
Glutamine-hydrolyzing asparagine synthetase is also commonly designated cell cycle control protein TS11. Asparagine synthetase plays an important role in the amino-acid biosynthesis pathway and is also important for L-asparagine biosynthesis. Via the L-glutamine route, it is involved in the synthesis of L-asparaine from L-aspartate. The protein contains one asparagine synthetase domain and one type-2 glutamine amidotransferase domain. The cell-cycle regulated gene encoding for asparagine synthetase, ts11, is necessary for G1 progression. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
ASNS polyclonal antibody detects endogenous levels of ASNS protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of Asparagine synthetase on different lysates using anti-Asparagine synthetase antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Human skeletal muscle
• 
  ICC staining Asparagine synthetase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---