Product Name :
Bag3 polyclonal antibody Background :
The Bag family of proteins are characterized by the presence of a 45 amino acid Bag domain through which they bind with high affinity to the ATPase domain of HSP 70, thereby negatively regulating HSP 70 chaperone activity. Bag-3 (Bcl-2-associated athanogene 3), also known as BIS or CAIR-1, is a 575 amino acid protein that contains one C-terminal Bag domain and two N-terminal WW domains. Like other members of the Bag family, Bag-3 functions to inhibit the chaperone activity of HSP 70, specifically by promoting the release of HSP 70-bound substrates. Additionally, Bag-3 exhibits anti-apoptotic activity via cell cycle control, suggesting a possible role for Bag-3 in tumor progression. The gene encoding Bag-3 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
Bag3 polyclonal antibody detects endogenous levels of Bag3 protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of Bag3 on Hela (1) and MCF-7 (2) cell using anti-Bag3 antibody at 1/1,000 dilution.
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  Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Bag3 antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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