Product Name :
BCHE polyclonal antibody Background :
Present in most cells except erythrocytes, butyrylcholine esterase (BChE), also designated acylcholine acylhydrolase or pseudocholinesterase, has esterase activity as well as aryl acylamidase activity. It hydrolyzes acylcholine into choline and carboxylate. BChE is synthesized in the liver and is highly reactive with organophosphate esters. BChE can form a homotetramer composed of two dimers linked by a disulfide bond. Defects in the gene encoding BChE are associated with the disease hypocholinesterasemia. Inhibition of BChE effects the toxicity of organophosphates in the respiratory system suggesting that BChE may play a role in respiratory function. In addition, BChE may play an important pharmocological role by hydrolyzing toxic esters. This suggests an involvement of BChE in a treatment for intoxication with substances such as cocaine. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
BCHE polyclonal antibody detects endogenous levels of BCHE protein. Immunogen :
Recombinant protein within Human BCHE aa 300-500. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of BCHE on human blood plasma using anti-BCHE antibody at 1/1,000 dilution.
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  ICC staining BCHE in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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