Product Name :
CD3 epsilon polyclonal antibody Background :
CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) These chains associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. Specificity :
CD3 epsilon polyclonal antibody detects endogenous levels of CD3 epsilon protein. Immunogen :
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal residues of CD3 epsilon. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of CD3E on different cell lysates using anti-CD3E antibody at 1/1000 dilution.
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  ICC staining CD3E in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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