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DAP12 polyclonal antibody BS90391
  • Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.
Product NameDAP12 polyclonal antibody
Catalog No.BS90391
Swiss-ProtO43914(Human)
Host Rabbit
ReactivityHuman
ApplicationsWB IHC FC
Application_allWB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100
BiowMW12 kDa
Alternative NameKAR-associated protein, TYROBP, DAP12, KARAP, DAP 12, DAP12, DNAX activation protein 12, DNAX-activation protein 12, KAR-associated protein, KARAP, Killer activating receptor associated protein, Killer-activating receptor-associated protein, PLOSL, TYOBP, TYRO protein tyrosine kinase binding protein, TYRO protein tyrosine kinase-binding protein, TYROBP,
Purification&PurityProA affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
DAP12 polyclonal antibody
Background :
Natural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity :
DAP12 polyclonal antibody detects endogenous levels of DAP12 protein.
Immunogen :
Synthetic peptide within human DAP12 aa 60-100.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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