EpCAM polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
EpCAM polyclonal antibody Background :
The epithelial cell adhesion molecule Ep-CAM, which is also designated tumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a major form and a minor form, which are reduced upon treatment with the amino-glycosylation inhibitor tunicamycin. Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumors. Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteine poor region, which is necessary for the adhesion properties of the molecule. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
EpCAM polyclonal antibody detects endogenous levels of EpCAM protein. Immunogen :
Synthetic peptide within human EpCAM 100-150 aa. Conjugate :
Unconjugated Modification :
Unmodification
EpCAM polyclonal antibody Background :
The epithelial cell adhesion molecule Ep-CAM, which is also designated tumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a major form and a minor form, which are reduced upon treatment with the amino-glycosylation inhibitor tunicamycin. Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumors. Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteine poor region, which is necessary for the adhesion properties of the molecule. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
EpCAM polyclonal antibody detects endogenous levels of EpCAM protein. Immunogen :
Synthetic peptide within human EpCAM 100-150 aa. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot analysis of EpCAM on mouse colon tissue lysate using anti-EpCAM antibody at 1/500 dilution. -
ICC staining EpCAM in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.