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Ephrin B2 polyclonal antibody BS90467
  • Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution. Positive control: Lane1: Human kidney Lane2: Mouse lung Lane3: Rat brain
  • ICC staining Ephrin B2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product NameEphrin B2 polyclonal antibody
Catalog No.BS90467
Swiss-ProtP52799(Human) P52800(Mouse)
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB ICC/IF
Application_allWB:1:500-1:1,000 ICC:1:50-1:200
BiowMW50 kDa
Alternative NameLERK-5, HTK-L, EFNB2, EPLG5, HTKL, LERK5, EFN B2, EFNB 2, Efnb2, EFNB2, Eph related receptor tyrosine kinase ligand 5, EPH-related receptor tyrosine kinase ligand 5, ephrin B2, Ephrin-B2, EphrinB2, EPLG 5, EPLG5, Htk L, HTK ligand, HTK-L, HTKL, LERK 5, LERK-5, LERK5, Ligand of eph related kinase 5, MGC126226, MGC126227, MGC126228, OTTMUSP00000024973,
Purification&PurityProA affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
Ephrin B2 polyclonal antibody
Background :
Ephrin-B and are conserved cytoplasmic tyrosine residues that are Eph receptors and ephrins optimistic supporting expression in many cases during embryogenesis and ephrin-B proteins may occur in expression domain interfaces. The transmembrane ligand ephrin-B2 and its receptor tyrosine Order a copy of this thesis Bidirectional signals mediated by both proteins play an important role in vascular development. Ephrin-B2 is essential for the normal morphogenesis of the embryonic vasculature and is angiogenic in tumors. It has been Identified as an important target of chemotheiples treatments.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :
Ephrin B2 polyclonal antibody detects endogenous levels of Ephrin B2 protein.
Immunogen :
recombinant protein
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution. Positive control: Lane1: Human kidney Lane2: Mouse lung Lane3: Rat brain
  • ICC staining Ephrin B2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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