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ERK1/2 polyclonal antibody BS90472
  • Western blot analysis of ERK1/2 on different cell lysates using anti-ERK1/2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: SW480 Lane 3: HCT116 Lane 4: PC12
  • ICC staining ERK1/2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product NameERK1/2 polyclonal antibody
Catalog No.BS90472
Swiss-ProtP27361(Human) P28482(Human) P63085(Mouse) Q63844(Mouse) P21708(Rat) P63086(Rat)
Host Rabbit
ReactivityHuman, Mouse, Rat, zebrafish
ApplicationsWB ICC/IF IP FC
Application_allWB:1:1,000-1:5,000 ICC:1:50-1:200 FC:1:50-1:100
BiowMW42/44 kDa
Alternative NameMAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK, ERK 1, ERK 2, ERK-2, ERK1, erk1/2, ERK2, ERT1, ERT2, Extracellular signal regulated kinase 1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Mapk1, MAPK2, MAPK3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01, p38, p40, p41, p42-MAPK, PRKM 2,
Purification&PurityProA affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
ERK1/2 polyclonal antibody
Background :
Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :
ERK1/2 polyclonal antibody detects endogenous levels of ERK1/2 protein.
Immunogen :
recombinant protein
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of ERK1/2 on different cell lysates using anti-ERK1/2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: SW480 Lane 3: HCT116 Lane 4: PC12
  • ICC staining ERK1/2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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