Product Name :
Fibrillarin polyclonal antibody Background :
Fibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
Fibrillarin polyclonal antibody detects endogenous levels of Fibrillarin protein. Immunogen :
Synthetic peptide of C-terminal human Fibrillarin. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of Fibrillarin on different cell lysates using anti-Fibrillarin antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: HepG2 Lane3: 293T Lane4: NIH-3T3
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  Flow cytometric analysis of HL-60 cells with Fibrillarin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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